Flow-Batch Sample Preparation for Fractionation of the Stress Signaling Phytohormone Salicylic Acid in Fresh Leaves

Table 3

Contents of free and total SA determined by the proposed and reference procedures (n = 3).

Samples	Species	Content (mg kg^−1)		F-values
		Proposed	Reference [16]
Sugarcane leaves	Free SA	0.29 ± 0.05	0.30 ± 0.02	6.2
	Total SA	0.38 ± 0.01	0.31 ± 0.01	1.0
Corn leaves	Free SA	0.18 ± 0.03	0.16 ± 0.02	2.2
	Total SA	0.89 ± 0.04	0.9 ± 0.1	6.2
Soybean leaves	Free SA	11.4 ± 0.3	11 ± 1	11
	Total SA	12 ± 1	11.2 ± 0.3	11

F_critical (95% confidence level): 19.