Simultaneous Determination of Multiple Active Components from Bushen Pills and Application in a Pharmacokinetic Study in Rats

Table 2

Matrix effect, extraction recovery, accuracy, and precision of PF, BBR, and IS in rat plasma.

Analyte	Spiked conc. (ng/mL)	Matrix effect		Extraction recovery		Intraday (n = 5)		Interday (n = 15)
		Mean ± SD (ng/mL)	RSD (%)	Mean ± SD (ng/mL)	RSD (%)	Accuracy (RE, %)	Precision (RSD, %)	Accuracy (RE, %)	Precision (RSD, %)
PF	6	102.23 ± 5.18	5.07	43.73 ± 1.49	3.40	−3.83	1.74	−4.87	7.03
	40	101.32 ± 4.45	4.39	45.67 ± 2.49	5.45	−3.67	2.86	−5.01	3.43
	400	101.89 ± 2.63	2.58	45.32 ± 2.25	4.97	2.71	4.08	3.44	4.16
BBR	0.2	101.88 ± 4.23	4.15	41.79 ± 2.95	7.05	−3.40	5.60	−4.50	8.83
	4.0	104.15 ± 1.57	1.51	38.47 ± 0.75	1.96	3.05	3.03	3.79	5.00
	16.0	100.21 ± 1.81	1.80	37.78 ± 0.71	1.89	−1.96	3.78	−2.27	3.82
IS	5	97.37 ± 3.21	3.30	45.00 ± 2.03	4.52	—	—	—	—