A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect”

Table 1

Osteocalcin concentrations in serum samples after several dilutions.

Dilution	Sample 1	Sample 2	Sample 3
	ng/mL
None	63.5	96.5	67.8
1 : 1	85.6	154.7	113.4
1 : 3	74.1	141.1	102.2
1 : 5	51.4	90.1	69.1
1 : 7	29.8	56.5	42.3
1 : 15	16.2	32.0	23.6