Research Article

A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect”

Table 4

Analytical recovery.

BGP concentration (ng/mL)
SamplesAdded concentrationTestedExpectedRecovery (%)

10.0019.9821.03105.26
15.6335.5734.9398.20
31.2549.3949.95101.13
62.5081.4283.48102.53
125.00136.48144.23105.68

2044.7645.80102.32
15.6359.1861.04103.14
31.2578.3275.6096.53
62.50105.01105.39100.36
125.00157.39168.64107.15