Estimation of the Content of Selected Active Substances in Primary and Secondary Herbal Brews by UV-VIS and GC-MS Spectroscopic Analyses

Table 1

Comparison of the content of dominant components in essential oils acquired from the primary and waste plant material.

Component	RI	RILit	Most fragment ions with RInt (%)	Primary material	Content (%)
					Waste material after the maceration process
					5 min	10 min	15 min	30 min
Peppermint
Menthone	1149	1148	112 (100%),; 139 (79%),; 81 (64%)	14.02^c ± 0.33	13.82^c±0.16	14.92^b ± 0.13	15.30^a ± 0.11	15.57^a ± 0.17
Menthol	1168	1167	71 (100%),; 41 (92%),; 81 (88%)	41.72^a ± 0.57	41.12^a ± 0.18	39.37^a ± 0.43	39.15^b ± 0.21	38.76^b ± 0.27
Menthyl acetate	1295	1294	43 (100%),; 95 (82%),; 81 (65%)	10.65^c ± 0.28	13.88^b ± 0.09	12.61^b ± 0.10	12.58^b ± 0.13	12.35^a ± 0.09
Chamomile
α-Bisabolol oxide B	1650	1656	43 (100),; 105 (42),; 143 (38)	10.01^c ± 0.50	10.96^b ± 0.05	11.45^b ± 0.24	11.06^b ± 0.31	13.43^a ± 0.26
α-Bisabolone oxide A	1677	1684	43 (100),; 93 (74),; 67 (44)	6.80^d ± 0.15	7.59^c ± 0.14	8.39^b ± 0.11	7.73^c ± 0.21	10.77^a ± 0.29
α-Bisabolol oxide A	1740	1748	238 (2),; 43 (100),; 143 (58),; 93 (29)	50.89^cd ± 1.43	52.82^b ± 0.58	49.68^d ± 0.45	54.83^a ± 0.29	52.19^bc ± 0.59
Fennel
Fenchone	1079	1083	152 (1),; 81 (100%),; 69 (52%),; 41 (31%)	14.26^b ± 0.04	14.16^c ± 0.09	14.74^a ± 0.04	14.10^c ± 0.01	13.94^d ± 0.01
trans-Anethole	1280	1282	148 (100%),; 147 (44%),; 117 (29%)	76.69^a ± 0.05	76.17^b ± 0.19	76.15^b ± 0.27	75.77^c ± 0.07	75.85^c ± 0.04
Lavender
Linalool	1094	1095	154 (1),; 71 (100%),; 43 (87),; 93 (69%)	26.46^d ± 0.10	28.41^a ± 0.21	27.57^b ± 0.17	26.96^c ± 0.07	26.54^d ± 0.19
Linalool acetate	1255	1254	93 (100%),; 43 (90%),; 41 (56%)	19.03^a ± 0.40	16.29^b ± 0.11	15.84^c ± 0.13	15.35^d ± 0.03	14.88^e ± 0.14
Sage
cis-Thujone	1098	1101	152 (4%),; 81 (100%),; 41 (85%),; 67 (60%)	16.31^a ± 0.27	15.87^ab ± 0.27	15.53^b ± 0.25	14.69^c ± 0.36	13.92^d ± 0.27
trans-Thujone	1109	1112	152 (2%),; 81 (100%),; 41 (98%),; 67 (72%)	8.36^a ± 0.05	8.33^a ± 0.08	8.37^a ± 0.05	8.00^b ± 0.18	7.86^b ± 0.14
Camphor	1141	1141	152 (4%),; 95 (100%),; 81 (54%),; 108 (46%)	16.57^a ± 0.35	14.44^b ± 0.15	13.81^c ± 0.18	12.78^d ± 0.28	11.53^e ± 0.23
Viridiflorol	1589	1592	222 (1%),; 43 (100%),; 109 (55%),; 69 (43%)	10.10^e ± 0.16	12.02^d ± 0.11	13.97^c ± 0.04	14.70^b ± 0.04	15.44^a ± 0.13
Caraway
Limonene	1021	1024	136 (17%),; 68 (100%),; 93 (60%),; 67 (43%)	47.88^a ± 0.21	47.61^a ± 0.23	40.22^d ± 0.20	47.07^b ± 0.14	43.17^c ± 0.16
Carvone	1236	1239	150 (9%),; 82 (100%),; 54 (49%),; 93 (34%)	46.80^e ± 0.15	47.20^d ± 0.13	48.17^c ± 0.11	48.89^b ± 0.11	53.05^a ± 0.19

RI: retention indices, RI_Lit: retention indices taken from the literature [13], and R_Int (%): relative intensities. Data are shown as mean ± SD. Different letters (a, b, c, etc.) in the line show a significant difference with.