Research Article
Development of a Chemiluminescence Immunoassay for Quantification of 25-Hydroxyvitamin D in Human Serum
| Samples | VD concentration (ng/mL) | Added concentration | Tested | Expected | Recovery (%) |
| 1 | 0.00 | 9.76 | 10.54 | 107.99 | 5.63 | 15.88 | 15.48 | 97.48 | 11.25 | 20.98 | 19.90 | 94.85 | 22.50 | 31.18 | 31.97 | 102.53 | 45.00 | 58.99 | 54.99 | 93.22 |
| 2 | 0 | 23.53 | 24.56 | 104.38 | 5.625 | 29.59 | 29.21 | 98.72 | 11.25 | 35.10 | 34.87 | 99.34 | 22.5 | 47.20 | 45.76 | 96.95 | 45 | 65.29 | 67.90 | 104.00 |
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