Pharmacokinetic Comparisons of Different Combinations of Yigan Jiangzhi Formula in Rats: Simultaneous Determination of Fourteen Components by UPLC-MS/MS

Table 6

Stability of the fourteen analytes in rat plasma under different conditions (mean ± SD, n = 6).

No.	Analyte	Conc. (ng/mL)	25°C for 4 h		4°C for 24 h		Three freeze-thaw cycles		−80°C frozen for 30 days
			Acc. (%)	Prec. (%)	Acc. (%)	Prec. (%)	Acc. (%)	Prec. (%)	Acc. (%)	Prec. (%)
1	Daidzein	0.5	4.78	4.63	−3.75	6.67	2.59	2.96	3.27	3.46
		10	1.57	2.49	1.43	7.78	−1.60	6.25	1.69	5.90
		80	−4.09	6.11	2.46	8.30	−2.36	9.06	2.35	8.54
2	Fermononetin	1.25	−2.79	3.63	1.35	7.52	2.94	4.82	−3.15	1.25
		25	2.88	6.10	3.09	4.52	2.09	7.39	1.32	5.28
		200	2.67	4.54	−2.81	5.65	4.20	7.94	5.25	1.87
3	Apigenin	0.5	4.04	6.67	1.58	4.49	0.96	2.17	3.06	6.87
		10	−2.43	4.84	−2.84	4.45	−1.60	6.25	3.25	2.38
		80	−3.10	3.15	−3.77	3.60	−5.06	8.28	4.68	3.26
4	Luteolin	2.5	1.77	6.67	−1.33	1.68	1.33	4.67	−2.16	3.76
		50	2.90	4.85	0.71	2.12	6.25	2.19	4.42	3.67
		400	1.44	3.00	1.26	4.10	1.37	2.06	1.88	2.74
5	Berberine	0.5	−3.49	6.67	3.03	6.25	4.16	5.05	2.79	5.61
		10	2.57	4.58	−2.42	10.02	−3.63	4.27	2.11	3.38
		80	2.44	4.19	−2.60	3.98	−4.35	3.03	3.70	5.49
6	Jatrorrhizin	0.5	2.22	2.78	−3.67	6.67	3.33	5.04	6.39	8.25
		10	−1.10	1.99	0.57	2.48	2.56	3.21	−3.73	4.81
		80	−4.07	7.42	−1.57	1.02	−2.13	1.57	3.28	9.34
7	Palmatine	0.5	1.39	6.67	4.72	2.62	2.33	5.36	−2.58	5.71
		10	−2.96	4.02	−1.39	5.33	0.83	2.31	3.58	2.19
		80	−1.07	4.94	−2.16	4.69	−2.04	8.06	4.01	11.64
8	Puerarin	5	4.45	12.43	3.13	3.97	3.21	7.33	1.59	6.28
		100	−1.81	5.79	−2.68	6.31	3.38	4.19	2.11	5.20
		800	−2.97	7.64	−3.75	9.18	−1.96	8.57	3.51	7.29
9	Ononin	1.25	1.33	5.37	4.00	4.84	1.33	2.83	1.26	4.43
		25	−2.84	2.70	−2.94	6.04	−1.81	8.44	−1.57	4.91
		200	−2.71	7.91	−5.66	6.39	−2.99	8.46	5.05	4.78
10	Calycosin-7-O-β-D-Glc	0.5	−1.71	2.11	2.93	3.98	5.15	10.04	−4.06	5.75
		10	−5.46	9.60	−4.49	13.92	−4.79	2.77	3.71	3.74
		80	−3.13	7.42	−4.41	8.13	−3.03	6.38	3.84	6.32
11	Tanshinol	25	−2.80	3.11	−5.33	8.80	−3.31	9.84	−7.67	10.91
		500	−3.08	8.89	2.34	1.27	−2.36	4.79	−4.47	4.05
		4000	−2.12	8.35	−5.65	11.94	−3.87	5.09	−5.03	6.53
12	Rosmarinic acid	1.25	−4.00	5.93	−4.83	9.42	−5.33	7.49	−1.33	5.97
		25	−2.50	9.19	−1.62	3.66	−3.27	5.46	−4.57	7.32
		200	2.92	7.10	5.33	10.16	3.32	6.77	−1.43	2.35
13	Alkanoic acid	5	−2.00	10.85	3.33	13.03	−2.67	9.79	−3.52	5.79
		100	−5.79	13.22	−2.14	2.89	−4.88	3.81	−3.33	5.67
		800	−2.05	7.84	−3.99	9.35	−1.36	7.32	−3.80	5.13
14	Salvianolic acid B	2.5	−1.33	9.40	−2.17	12.67	−2.70	9.33	−2.67	4.24
		50	−3.04	10.25	−1.30	5.32	−1.86	2.41	−5.06	7.65
		400	−2.68	6.47	−3.36	4.13	−3.72	3.40	−2.68	7.22