Establishment of the Quantitative Analysis of Multiindex in Euphorbia lathyris by the Single Marker Method for Euphorbia lathyris Based on the Quality by Design Concept

Table 1

PBD experimental program and results.

No.	Methanol ratio A (%)	Flow rate B (mL/min)	Column temperature C (°C)	Sample injection volume D (μL)	Chromatographic resolution Y
1	60 (−1)	1.2 (+1)	20 (−1)	5 (−1)	1.62
2	70 (+1)	1.2 (+1)	30 (+1)	5 (−1)	0.89
3	60 (−1)	0.8 (−1)	30 (+1)	15 (+1)	1.53
4	60 (−1)	1.2 (+1)	30 (+1)	15 (+1)	0.85
5	60 (−1)	0.8 (−1)	20 (−1)	15 (+1)	3.23
6	70 (+1)	0.8 (−1)	30 (+1)	15 (+1)	0.78
7	70 (+1)	1.2 (+1)	20 (−1)	15 (+1)	1.45
8	70 (+1)	0.8 (−1)	20 (−1)	5 (−1)	2.88
9	70 (+1)	0.8 (−1)	30 (+1)	5 (−1)	0.91
10	60 (−1)	0.8 (−1)	20 (−1)	5 (−1)	3.12
11	70 (+1)	1.2 (+1)	20 (−1)	15 (+1)	0.86
12	60 (−1)	1.2 (+1)	30 (+1)	5 (−1)	1.94