Establishment of the Quantitative Analysis of Multiindex in Euphorbia lathyris by the Single Marker Method for Euphorbia lathyris Based on the Quality by Design Concept

Table 2

CCD experimental program and response value.

No.	Methanol ratio X1 (%)	Column temperature X2 (°C)	Chromatographic resolution Y
1	65 (0)	25 (0)	1.56
2	65 (0)	25 (0)	1.12
3	65 (0)	25 (0)	0.98
4	72.07 (1.414)	25 (0)	1.33
5	65 (0)	17.93 (−1.414)	3.19
6	65 (0)	25 (0)	0.86
7	65 (0)	25 (0)	1.11
8	60 (−1)	20 (−1)	1.87
9	65 (0)	32.07 (1.414)	1.96
10	60 (−1)	30 (+1)	2.08
11	70 (+1)	20 (−1)	1.84
12	57.93 (−1.414)	25 (0)	2.35
13	70 (+1)	30 (+1)	0.19