Establishment of the Quantitative Analysis of Multiindex in Euphorbia lathyris by the Single Marker Method for Euphorbia lathyris Based on the Quality by Design Concept

Table 8

Relative retention time measured by different brands of instruments and chromatographic columns.

Instrument	Chromatographic columns	Relative retention time
		Euphorbia factor L3/euphorbia factor L1	Euphorbia factor L4/euphorbia factor L1
UltiMate 3000	Sagix Copsil C18	0.5591	1.1251
UltiMate 3000	ZORBAX SB-C18	0.5671	1.1302
Waters 2695	Sagix Copsil C18	0.5904	1.1260
Waters 2695	GL InertSustain C18	0.5681	1.1311
Agilent 1290 Infinity II	ZORBAX SB-C18	0.5802	1.1265
Agilent 1290 Infinity II	GL InertSustain C18	0.5719	1.1235
Average		0.5728	1.1271
RSD%		1.92	0.26