Development of Simple Analytical Method for B-Group Vitamins in Nutritional Products: Enzymatic Digestion and UPLC-MS/MS Quantification

Table 2

Investigation of mobile phase programs for the determination of B-group vitamins in this study.

Program	Time (min)	%A	%B
Isocratic	0–5.0	50	50
Gradient 1	0–0.5	99	1
	2.5	92	8
	5.0	10	90
	6.0	10	90
	6.1–10.0	99	1
Gradient 2	0–0.5	92	8
	2.5	80	20
	3.0	50	50
	6.0	10	90
	7.0–10.0	95	5
Gradient 3	0–0.1	99	1
	0.5	95	5
	2.5	92	8
	4.0–5.0	10	90
	6.0–10.0	99	1