Research Article
Development of Simple Analytical Method for B-Group Vitamins in Nutritional Products: Enzymatic Digestion and UPLC-MS/MS Quantification
Table 3
MS/MS parameters for the determination of B-group vitamins in this study (bold numbers indicate quantitative ions).
| Compound | Precursor ion (m/z) | Product ion (m/z) | Cone voltage (V) | Collision energy (eV) |
| THI | 265 | 81 | 20 | 30 | | 122 | 24 | 34 |
| 13C4-THI | 269 | 81 | 24 | 28 | | 122 | 22 | 16 |
| RIB | 377 | 198 | 34 | 38 | | 243 | 34 | 22 |
| 13C415N2-RIB | 383 | 175 | 44 | 38 | | 249 | 44 | 22 |
| NIC | 123 | 80 | 44 | 16 | | 96 | 44 | 18 |
| 2H4-NIC | 127 | 84 | 44 | 18 | | 100 | 44 | 18 |
| NIA | 124 | 80 | 40 | 20 | | 106 | 40 | 20 |
| 2H4-NIA | 128 | 84 | 44 | 16 | | 109 | 44 | 16 |
| PYN | 170 | 134 | 30 | 20 | | 152 | 30 | 13 |
| 13C4-PYN | 174 | 138 | 28 | 20 | | 156 | 28 | 12 |
| PYL | 168 | 94 | 24 | 22 | | 150 | 24 | 12 |
| 2H3-PYL | 171 | 97 | 22 | 24 | | 153 | 22 | 12 |
| PYM | 169 | 134 | 24 | 20 | | 152 | 24 | 12 |
| 2H3-PYM | 172 | 136 | 26 | 20 | | 155 | 26 | 14 |
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