Development of Simple Analytical Method for B-Group Vitamins in Nutritional Products: Enzymatic Digestion and UPLC-MS/MS Quantification

Table 4

Retention times (min) of B-group vitamins obtained by different mobile phase programs.

Compound	Isocratic	Gradient 1	Gradient 2	Gradient 3
THI	2.02	5.21	4.54	5.14
RIB	2.47	5.77	4.99	5.79
NIC	2.14	5.34	4.60	5.27
NIA	2.09	3.54	2.56	3.36
PYN	2.15	5.22	4.45	4.97
PYL	2.14	4.71	3.72	4.26
PYM	1.93	2.76	1.81	2.74