Development and Validation of Ecofriendly HPLC-MS Method for Quantitative Assay of Amoxicillin, Dicloxacillin, and Their Official Impurity in Pure and Dosage Forms

Table 6

Statistical analysis of the proposed HPLC/MS method and the reported HPLC method for the determination of AMOX and DIC in pharmaceutical formulations.

Parameters	AMOX, HPLC/MS	DIC, HPLC/MS	Reported HPLC method [33]
			AMOX	DIC
Mean	99.63	102.65	100.03	102.80
SD	1.237	1.297	1.568	1.222
Variance	1.530	1.683	2.460	1.493
N	6	6	6	6
Student's t-test(2.228)	0.633	0.835	ـــــ	ـــــ
F-test(5.050)	1.608	1.127	ـــــ	ــــــ

The values between parenthesis are corresponding to the theoretical values of t and F (). HPLC determination of AMOX in binary mixture with DIC using acetonitrile: 1% acetic acid solution (47 : 53, vol/vol) as mobile phase and UV detection at 240 nm [33].