Development of Attenuated Total Reflectance Mid-Infrared (ATR-MIR) and Near-Infrared (NIR) Spectroscopy for the Determination of Resistant Starch Content in Wheat Grains

Table 3

Develop and screening for the best calibration model for resistant starch in wheat grain samples using ATR-MIR and NIR spectra.

Spectroscopy	Preprocessing methods	Calibration		Internal cross-validation
			RMSEC		RMSEP	RPD
NIR	Original	0.641	0.403	0.482	0.493	1.363
	MN	0.673	0.384	0.526	0.472	1.424
	MN + MSC	0.671	0.385	0.523	0.474	1.418
	MN + SGS	0.672	0.385	0.552	0.459	1.464
ATR-MIR	Original	0.922	0.188	0.804	0.303	2.218
	Baseline	0.937	0.169	0.828	0.284	2.366
	Baseline + GFS	0.935	0.171	0.826	0.286	2.35
	Baseline + SGS	0.935	0.171	0.825	0.287	2.341

, determination coefficient of calibration; , determination coefficient of prediction; RMSEC, root mean square error of calibration; RMSEP, root mean square error of prediction; RPD, residual predictive deviation; MN, mean normalization; MSC, multiplicative scatter correction; SGS, Savitzky–Golay smoothing; GFS, Gaussian filter smoothing.