Sensitive Quantification of Liensinine Alkaloid Using a HPLC-MS/MS Method and Its Application in Microvolume Rat Plasma

Table 5

Summary of stability of QC samples of liensinine under different storage conditions (n = 3).

Conditions	Spiked concentration (ng/mL)	Recovery (%)	RSD (%)
12 h RT	0.05	98.10	2.3
	5.0	98.72	4.5
	750	101.11	0.9
Autosampler 24 h	0.05	96.82	4.2
	5.0	100.17	7.7
	750	98.99	3.9
After 3 freeze-thaw cycles	0.05	99.64	0.8
	5.0	103.01	2.4
	750	99.03	0.9
−20°C for one month	0.05	92.57	5.5
	5.0	94.39	9.2
	750	95.12	2.8