Spectrum-Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Yangyin Tongnao Prescription
Table 4
The peak area of each common peak to all the samples.
Peak no.
tR (min)
Compounds
Mean peak area of each common peak (n = 3)
CV (%)
S1
S2
S3
S4
S5
S6
S7
S8
S9
Control
P1
4.35
Unknown
58.239
1598.968
1434.003
2242.623
1847.072
1928.97
1514.767
1870.101
1977.346
1608.01
37.13
P2
13.874
Unknown
894.038
124.294
106.241
153.788
127.161
149.786
112.926
141.261
149.642
217.682
110.10
P3
14.459
Unknown
158.758
485.685
410.196
602.872
477.105
570.061
429.368
537.156
520.098
465.7
26.48
P4
17.721
Unknown
2097.543
1797.045
1551.842
2169.958
1794.198
2087.783
1494.875
1850.784
1858.046
1622.726
12.73
P5
20.252
Vanillic acid
188.243
199.638
160.548
267.092
216.213
258.9
190.743
241.708
237.757
196.955
15.98
P6
23.059
Unknown
98.432
268.577
247.529
487.828
425.278
529.692
497.381
759.812
755.699
452.248
46.47
P7
23.891
Puerarin
12356.807
11137.137
9685.376
12278.256
10341.828
12285.794
9020.642
10978.242
10895.806
10997.765
10.16
P8
26.192
Unknown
3068.374
2724.584
2365.726
3034.727
2546.809
3021.683
2213.586
2713.51
2681.596
2472.933
10.92
P9
27.737
Unknown
2692.924
2497.148
2104.842
2746.072
2280.202
2812.948
2077.906
2645.69
2564.506
2491.36
10.39
P10
33.463
Unknown
451.595
431.389
415.448
893.588
757.391
812.497
767.16
924.589
988.359
715.78
29.60
P11
37.292
Unknown
55.412
208.42
138.712
237.401
195.599
230.8
189.65
238.356
233.033
191.932
29.67
P12
38.998
Unknown
118.244
134.402
218.107
382.948
320.283
391.466
352.623
444.97
479.304
315.816
39.00
P13
39.844
Ferulic acid
537.599
227.175
341.181
481.695
367.531
450.176
324.529
400.558
437.673
396.457
22.17
P14
44.968
Unknown
53.301
210.321
153.735
280.818
177.932
210.875
185.905
233.502
270.171
174.027
33.13
P15
46.175
Unknown
119.763
155.493
123.31
252.22
161.781
170.748
149.025
186.218
221.438
171.111
23.92
P16
46.762
Unknown
318.118
175.192
120.313
183.771
131.151
157.711
134.175
161.501
147.948
169.987
32.89
P17
47.891
Unknown
567.238
1400.02
1197.264
1678.034
1391.706
1645.648
1264.935
1542.522
1496.576
1290.745
23.53
P18
48.897
Unknown
102.963
171.029
160.95
196.841
163.064
193.266
142.509
174.395
174.906
152.996
16.49
P19
50.891
Unknown
541.894
225.149
194.53
275.116
230.743
267.933
190.102
258.261
244.444
209.586
38.67
P20
52.074
Unknown
480.788
483.885
403.028
540.935
453.536
531.823
424.461
547.367
501.954
431.888
10.64
P21
56.112
Daidzein
1632.799
617.779
593.706
561.935
520.579
618.128
268.638
354.741
442.295
623.4
60.14
P22
58.608
Unknown
232.051
245.562
204.692
333.709
276.466
323.545
212.518
301.423
304.541
244.717
17.31
P23
64.372
Formononetin
218.719
179.679
178.056
174.758
174.97
173.96
174.36
173.419
171.243
155.605
8.96
P24
68.493
Unknown
200.638
237.3
214.887
266.736
211.177
245.117
209.784
207.319
239.623
225.842
9.26
CV (%) = σ/μ × 100; s was the standard deviation; μ was the average value of the peak area. Control represents the control fingerprint automatically generated by the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine.
