Determination of Sudan I and II in Food by High-Performance Liquid Chromatography after Simultaneous Adsorption on Nanosilica
Table 3
Quantification of Sudan I and II in food samples (mean ± confidence, n = 3).
Sample
Found Sudan I (µg·L−1)
Found Sudan II (µg·L−1)
Added Sudan I and Sudan II with same concentration (µg·L−1)
Recovery of Sudan I (%)
Recovery of Sudan II (%)
Dried meat 1
ND
ND
50,00
82.76 ± 1.13
83.64 ± 0.81
Dried meat 2
26.72 ± 1.48
47.68 ± 1.39
50,00
101.53 ± 1.23
93.24 ± 0.78
Dried meat 3
ND
ND
50,00
84.32 ± 2.25
83.90 ± 2.40
Snack 1
17.59 ± 0.80
44.43 ± 2.57
50,00
77.31 ± 1.06
81.73 ± 2.07
Snack 2
26.85 ± 0.45
40.78 ± 2.20
50,00
82.13 ± 1.03
82.20 ± 1.24
Snack 3
15.97 ± 0.38a
28.46 ± 0.43
50,00
86.82 ± 0.83
91.22 ± 1.46
Snack 4
ND
ND
50,00
84.98 ± 0.99
86.90 ± 2.31
Snack 5
13.23 ± 0.18b
20.39 ± 1.10
50,00
86.49 ± 1.15
86.34 ± 0.99
ND: not detectable. aAfter desorption, the solution was diluted fivefold with the mobile phase because the contents of Sudan II in spiked snack sample 3 were outside the linear range. bAfter desorption, the solution was diluted 10-fold with the mobile phase because the contents of Sudan I and Sudan II in spiked snack sample 5 were outside the linear range.
