Research Article

A Direct and Sensitive Method for Determination of 5-Fluorouracil in Colorectal Cancer Cells: Evaluating the Effect of Stromal Cell on Drug Resistance of Cancer Cells

Table 1

Mass spectrometric parameters of analyte and internal standard (IS).

AnalytesSRM transition m/z (Q1 ⟶ Q3)Fragmentor (V)CE (eV)Retention time (min)

5-FU129.0 ⟶ 42.290171.919
5-BrU188.8 ⟶ 41.890212.228