Simultaneous Quantification of Pioglitazone and Omarigliptin in Rat Plasma by UHPLC-MS/MS and Its Application to Pharmacokinetic Study after Coadministration of the Two Drugs

Table 3

Precision and accuracy for the analysis of pioglitazone and omarigliptin in rat plasma (n = 6).

		Intraday			Interday
Compound	Nominal conc.	Measured conc.	Precision	Accuracy	Measured conc.	Precision	Accuracy
	(ng/mL)	(Mean ± SD)	(RSD, %)	(RE, %)	(Mean ± SD)	(RSD, %)	(RE, %)
Pioglitazone	5	5.03 ± 0.09	1.76	−1.59	5.40 ± 0.47	8.62	7.96
	15	15.39 ± 1.17	7.59	−4.84	15.61 ± 1.08	6.93	4.06
	400	366.39 ± 1.36	0.37	−8.68	366.59 ± 1.18	0.32	−8.35
	1500	1439.91 ± 4.68	0.33	−4.09	1437.11 ± 5.49	0.38	−4.19
Omarigliptin	10	9.92 ± 0.17	1.75	−0.77	10.51 ± 0.71	6.74	5.05
	30	32.00 ± 0.41	1.28	6.67	30.60 ± 1.69	5.53	2.00
	800	734.28 ± 2.88	0.39	−8.21	733.20 ± 9.05	1.23	−8.35
	3000	2947.16 ± 10.01	0.34	−1.76	2946.69 ± 12.01	0.41	−1.78