Journal of Analytical Methods in Chemistry

Research Article

A Study of the Identification, Fragmentation Mode and Metabolic Pathways of Imatinib in Rats Using UHPLC-Q-TOF-MS/MS

Table 1

Chromatographic retention times, precursor ions, and key fragments of IM and its metabolites in rat plasma, bile, urine, and feces sample.
No.Formula of [M + H]+ ionMS dataError (ppm)tR (min)MS/MS dataPlasmaBileUrineFecesNo.Formula of [M + H]+ ion
M1C29H30N7O3+524.24141.46.36119,231,392,506−+−−M1C29H30N7O3+
M2C35H40N7O8+686.29360.46.45217,410,510−++−M2C35H40N7O8+
M3C15H22N3O2+276.17080.76.78147,258−++−M3C15H22N3O2+
M4C29H30N7O3+524.2406−0.17.22203,231,392,410,506−++−M4C29H30N7O3+
M5C30H32N6O+492.25080.37.47217,275,392++++M5C30H32N6O+
M6C29H32N7O2+510.2611−0.28.19111,217,189,277,410+++−M6C29H32N7O2+
M7C29H32N7O2+510.261−0.48.81277,380,394,410++++M7C29H32N7O2+
M8C29H30N7O3+524.241219.1119,203,231,410−+−−M8C29H30N7O3+
M9C24H23N6O+411.19310.89.41134,247,264,394−+−−M9C24H23N6O+
M10C29H32N7O2+510.2611−0.29.46111,189,217,410+−++M10C29H32N7O2+
M11C18H17N5O+320.1498−2.59.5261,278++−+M11C18H17N5O+
M12C29H31N7O3+524.24090.69.7699,217,290,506++++M12C29H31N7O3+
M13C29H30N7O2+508.2447−1.610.08203,222,231,247,394++++M13C29H30N7O2+
M14C29H32N7O2+510.261−0.310.37111,217,410+−−−M14C29H32N7O2+
M15C28H30N7O+480.2505−0.210.52119,203,222,394++++M15C28H30N7O+
M16C29H29N7O2+508.2454−0.310.75175,203,222,231,247,000++−+M16C29H29N7O2+
M17C26H24N6O+437.2082−0.611.09160,290,379,394+−−−M17C26H24N6O+
M18C29H32N7O2+510.2598−2.911.54189,277,394++++M18C29H32N7O2+
M19C29H30N7O3+524.2397−1.811.54189,217,394,494++−−M19C29H30N7O3+
M20C24H20N5O3+426.15824.911.83133,277,408−++−M20C24H20N5O3+
M21C29H30N7O2+508.2443−2.512.06222,231,247,394++++M21C29H30N7O2+
M22C29H29N7O3+524.2396−2.112.42219,247,394,478+−+−M22C29H29N7O3+
M23C30H34N7O+508.2817−0.512.5119,189,217,264,408+−+−M23C30H34N7O+
M24C32H33N8O3S+609.2383−1.312.68171,215,394,478,565−+++M24C32H33N8O3S+
M25C24H20N5O3+426.15681.713.2149,261,408−+++M25C24H20N5O3+
M26C24H19N5O2+410.16130.414.17133,261,392+++−M26C24H19N5O2+
M27C24H21N5O+396.1818−0.615.7119,378+−−−M27C24H21N5O+