Single Standard Substance for the Simultaneous Determination of Eleven Components in the Extract of Paeoniae Radix Alba (Root of Paeonia lactiflora Pall.)
Table 9
Comparison of the concentrations (μg/mL) of the ten components determined by the external standard method (ESM) and the quantitative analysis of multicomponent with a single-marker (QAMS) method.
Component
Quantitative method/REa
RPLP from Pan’an
RPLP from Dongyang
RPLP from Baishan
RPLP from Hangzhou
PRA from Zhangzhongjing Pharmacy
PRA from Renshoutang
PRA from Qixiangge
PRA from Henan Herbal Pharmacy
RPLP from Pan’anb
RPLP from Dongyangb
RPLP from Baishanb
RPLP from Hangzhoub
Gallic acid
ESM
25.64
25.79
31.29
51.13
149.3
137.3
113.5
168.1
78.6
119.3
111.8
386.0
QAMS
25.06
25.09
30.68
50.54
150.0
138.5
113.8
168.7
79.91
122.4
113.3
387.4
RE (%)
−2.3
−2.7
−1.9
−1.2
0.5
0.8
0.2
0.4
1.6
2.6
1.3
0.4
Oxypaeoniflorin
ESM
120.4
143.2
99.61
99.60
78.73
70.71
77.87
78.13
119.7
129.4
79.30
106.2
QAMS
119.8
142.9
97.64
97.20
75.81
67.75
74.91
75.07
120.6
131.5
77.21
103.8
RE (%)
−0.5
−0.2
−2.0
−2.4
−3.7
−4.2
−3.8
−3.9
0.7
1.6
−2.6
−2.2
Catechin
ESM
276.2
607.1
61.22
65.54
137.9
118.5
123.1
152.2
/c
52.10
/
/
QAMS
286.8
631.0
60.92
65.14
140.8
121.2
125.4
155.6
/
52.51
/
/
RE (%)
3.8
3.9
−0.5
−0.6
2.2
2.2
1.9
2.2
/
0.8
/
/
Albiflorin
ESM
6183
7913
353.7
2950
1092
908.7
847.4
741.2
4673
4387
234.9
3830
QAMS
6251
7966
347.2
2953
1090
909.7
844.1
735.9
4782
4511
229.5
3829
RE (%)
1.1
0.7
−1.8
0.1
−0.1
0.1
−0.4
−0.7
2.3
2.8
−2.3
0.0
Ethyl gallate
ESM
/
/
63.09
70.59
/
/
/
/
/
/
/
/
QAMS
/
/
61.53
68.91
/
/
/
/
/
/
/
/
RE (%)
/
/
−2.5
−2.4
/
/
/
/
/
/
/
/
Galloylpaeoniflorin
ESM
154.3
131.3
98.35
131.7
158.8
129.5
133.8
165.8
75.69
66.02
78.02
181.2
QAMS
154.9
130.3
96.33
130.3
158.8
129.0
132.9
165.8
74.06
64.18
75.96
181.0
RE (%)
0.4
−0.7
−2.1
−1.1
0.0
−0.4
−0.7
0.0
−2.2
−2.8
−2.6
−0.1
Pentagalloylglucose
ESM
437.7
460.6
251.4
365.0
603.2
404.0
407.2
553.8
/
/
/
/
QAMS
444.9
465.9
255.8
368.4
609.0
410.6
412.0
558.5
/
/
/
/
RE (%)
1.7
1.2
1.7
0.9
1.0
1.6
1.2
0.8
/
/
/
/
Benzoic acid
ESM
338.6
572.8
112.0
111.6
/
/
/
/
1245
1231
765.3
658.1
QAMS
338.9
573.0
109.3
108.5
/
/
/
/
1271
1264
774.4
655.2
RE (%)
0.1
0.0
−2.3
−2.8
/
/
/
/
2.1
2.6
1.2
−0.4
Benzoylpaeoniflorin
ESM
335.0
368.7
268.8
274.1
149.3
118.8
150.4
165.8
102.3
154.3
110.1
129.7
QAMS
339.1
372.3
269.4
273.6
145.8
114.8
146.9
162.5
99.00
154.4
106.5
125.0
RE (%)
1.2
1.0
0.2
−0.2
−2.4
−3.4
−2.3
−2.0
−3.2
0.1
−3.3
−3.7
Paeonol
ESM
15.68
17.64
18.42
/
/
/
/
/
/
/
/
/
QAMS
15.04
16.96
17.77
/
/
/
/
/
/
/
/
/
RE (%)
−4.1
−3.8
−3.5
/
/
/
/
/
/
/
/
/
aRelative error (RE), calculated as (CQAMS − CESM)/CESM × 100%. bWater was used instead of 70% (v/v) ethanol for the extraction of these batches. c“/” means the content is below the LOD.
