Quantitation of Apremilast in Beagle Dogs Plasma by UPLC-MS-MS and Its Application to Pharmacokinetic Studies

Table 3

Pharmacokinetic parameters of apremilast after its single oral administration at 30 mg day⁻¹ in beagle dogs (mean ± SD, n = 5).

Pharmacokinetics parameters	Apremilast
AUC(0-t) ()	22650.98±
AUC(0-∞) ()	22758.56±
t1/2z (h)	5.41±
Tmax (h)	3.04±
Vz/F (L)	11.28±
CLz/F (L h^−1)	1.52±
Cmax (ug L^−1)	2148.33±

Blood samples (3.0 ml) were collected in heparinized tubes before dosing and at 0.17, 0.33, 0.50, 0.75, 1.00, 1.50, 2.00, 3.00, 4.00, 6.00, 9.00, 12.00, 24.00, 36.00, 48.00, 60.00, and 72.00 h postdosing, respectively. Plasma samples were obtained after immediate centrifugation of collected blood at 4000 rpm for 10 min.