Integrating Study on Qualitative and Quantitative Characterization of the Major Constituents in Shuanghuanglian Injection with UHPLC/Q-Orbitrap-MS and UPLC-PDA

Table 2

Collated results for linearity, LODs, LOQs, intra- and interday precisions, repeatability, stability, and recovery test of the 22 detected analytes in SHLI.

Analytes	Regression equations	r	Linear Range (µg/mL)	LOQs (µg/mL)	LODs (µg/mL)	Precision		Repeatability (RSD, %, n = 6)	Stability (RSD, %, n = 6)	Recovery Test
						Intraday (RSD, %; n = 6)	Interday (RSD, %; n = 3)			Recovery rate (%)	RSD (%)
Nea	y = 20099x − 428.07	0.999	0.5120–32.77	0.0569	0.0190	0.1	0.1	0.4	0.4	101.7	1.6
FoE	y = 1432.6x + 741.52	0.999	1.563–100.0	0.5208	0.1736	0.3	0.4	0.4	0.2	102.3	1.8
Cha	y = 21363x − 3658.0	0.999	0.6088–38.96	0.0676	0.0226	0.1	0.1	0.4	0.3	100.2	1.0
Cra	y = 9546.6x − 1111.6	0.999	0.5895–37.73	0.1965	0.0655	0.1	0.3	0.3	0.4	100.8	2.5
Caa	y = 15144x + 618.54	0.999	0.0945–6.048	0.0945	0.0315	0.7	3.4	1.5	2.7	105.4	1.9
Sea	y = 6601.9x + 461.97	0.999	0.5000–32.00	0.5000	0.1667	0.6	2.4	1.2	1.8	97.39	2.3
Seg	y = 11802x − 1412.3	0.999	0.2813–18.00	0.2813	0.0938	0.2	0.4	0.2	0.5	101.3	1.3
Iso	y = 10864x − 3542.2	0.999	1.167–74.66	0.1296	0.0432	0.1	0.1	0.4	0.3	102.2	1.7
Hyp	y = 10263 − 337.99	0.999	0.1037–6.636	0.1037	0.0346	0.3	0.4	0.4	0.7	102.7	1.0
Scu	y = 23346x − 2670.7	0.999	0.1813–11.60	0.0604	0.0202	0.2	0.2	1.0	0.5	109.3	1.3
FoA	y = 9905.5x − 3660.3	0.999	1.209–77.40	0.1344	0.0448	0.1	0.3	0.4	0.2	98.17	1.8
IaB	y = 20276x − 2334.8	0.999	0.2613–16.72	0.0871	0.0290	0.5	0.5	0.4	0.4	101.4	1.0
IaA	y = 24072x − 1409.8	0.999	0.09375–6.000	0.0938	0.0313	0.5	0.5	0.4	0.4	107.6	1.1
IaC	y = 23139x − 3039.9	0.999	0.2630–16.83	0.0877	0.0292	0.6	0.3	0.3	0.4	102.3	1.1
Pil	y = 4697.3x − 696.84	0.999	0.4345–27.81	0.4345	0.1448	1.0	0.3	0.8	0.7	95.06	2.3
Chg	y = 25004x − 2952.2	0.999	0.3444–22.04	0.1148	0.0383	0.1	0.2	0.4	0.2	98.76	2.7
Org	y = 23574x − 7113.5	0.999	0.8750–56.00	0.0972	0.0324	0.1	0.1	0.3	0.2	103.7	0.7
Oro	y = 29742x − 741.55	0.999	0.07225–4.624	0.0723	0.0241	0.5	0.5	1.2	0.7	103.1	2.0
Bai	y = 44049x − 6983.7	0.999	0.1581–10.12	0.1581	0.0527	0.3	3.5	1.5	1.0	107.5	2.2
Won	y = 46125x − 496.68	0.999	0.0147–0.9386	0.0147	0.0049	0.6	2.0	1.1	0.6	106.0	2.7
OrA	y = 48713x − 422.74	0.999	0.01291–0.8260	0.0129	0.0043	0.4	1.3	1.4	1.1	95.52	1.6
Bac	y = 26701x − 15617	0.999	1.875–120.0	0.0667	0.0222	0.1	0.2	0.5	0.3	100.7	3.0