Quantitation of Diclofenac, Tolbutamide, and Warfarin as Typical CYP2C9 Substrates in Rat Plasma by UPLC-MS/MS and Its Application to Evaluate Linderane-Mediated Herb-Drug Interactions

Table 3

Analytical curves, correlation coefficient (r), linear range, and LLOQ of diclofenac, tolbutamide, and warfarin

Compounds	Analytical curves	r	Linear range (μg/mL)	LLOQ (μg/mL)	RSD (%)	RE (%)
Diclofenac	y = 0.00210x + 0.00733	0.9998	0.020–5.0	0.020	10.91	0.0
Tolbutamide	y = 0.000252x + 0.0307	0.9973	0.200–100	0.200	3.47	5.0
Warfarin	y = 0.00271x + 0.0277	0.9977	0.050–20	0.050	4.24	3.2