Quantitation of Diclofenac, Tolbutamide, and Warfarin as Typical CYP2C9 Substrates in Rat Plasma by UPLC-MS/MS and Its Application to Evaluate Linderane-Mediated Herb-Drug Interactions

Table 4

Intraday/interday accuracy, precision, recovery, and matrix effect of diclofenac, tolbutamide, and warfarin in rat plasma.

Compounds	Added concentration (μg/mL)	Found concentration (μg/mL)	Intraday RSD (%)	Interday RSD (%)	Accuracy RE (%)	Recovery (%)	Matrix effect (%)
Diclofenac	0.060	0.06 ± 0.00	5.76	5.32	1.1	60.01 ± 3.35	95.30 ± 5.33
	0.40	0.40 ± 0.01	3.52	3.49	2.9	58.83 ± 1.83	96.07 ± 3.00
	3.8	3.74 ± 0.05	1.46	4.64	0.5	63.02 ± 1.16	96.43 ± 1.78
Tolbutamide	0.600	0.61 ± 0.03	4.86	3.70	−4.7	63.58 ± 2.27	99.03 ± 3.64
	4.00	4.21 ± 0.03	0.82	3.39	6.6	63.83 ± 2.60	103.70 ± 0.02
	75.0	79.12 ± 1.41	1.79	3.58	10	64.27 ± 2.47	103.70 ± 0.02
Warfarin	0.15	0.15 ± 0.00	3.02	6.27	2.7	63.84 ± 1.89	92.30 ± 2.73
	1.5	1.64 ± 0.03	1.56	2.02	10.3	61.17 ± 0.84	103.09 ± 1.41
	15	14.20 ± 0.14	1.00	2.08	−6.9	71.84 ± 1.09	99.88 ± 1.52