Tissue Distribution of Total Flavonoids Extracts of Drynariae Rhizoma in Young and Old Rats by UPLC–MS/MS Determination

Table 5

Interday and intraday precision and accuracy data for assays of astragalin in different tissues (n = 6).

Tissues	Spiked (ng/mL)	Interday			Intraday
		Mean ± SD	Precision (RSD, %)	Relative error (RE, %)	Mean ± SD	Precision (RSD, %)	Relative error (RE, %)
Heart	8.10	8.08 ± 1.07	13.24	−0.25	7.91 ± 0.93	11.76	−2.35
	40.50	38.56 ± 3.40	8.82	−4.79	37.90 ± 3.94	10.40	−6.42
	81.00	84.75 ± 8.92	10.53	4.63	83.60 ± 11.21	13.41	3.21
Liver	8.10	9.12 ± 1.20	13.16	12.59	9.08 ± 1.06	11.67	12.10
	40.50	37.17 ± 4.65	12.51	−8.22	36.06 ± 3.97	11.01	−10.96
	81.00	82.91 ± 5.22	6.30	2.36	82.43 ± 3.95	4.79	1.77
Spleen	5.05	4.55 ± 0.60	13.19	−9.90	4.61 ± 0.52	11.28	−8.71
	10.10	11.51 ± 1.47	12.77	13.96	11.42 ± 1.23	10.77	13.07
	25.25	24.75 ± 3.68	14.87	−1.98	22.94 ± 3.07	13.38	−9.15
Lung	8.10	8.78 ± 1.09	12.41	8.40	8.85 ± 1.24	14.01	9.26
	40.50	43.12 ± 4.18	9.69	6.47	42.70 ± 2.98	6.98	5.43
	81.00	85.32 ± 6.58	7.71	5.33	86.32 ± 5.75	6.66	6.57
Kidney	5.05	4.66 ± 0.64	13.73	−7.72	4.98 ± 0.51	10.24	−1.39
	10.10	10.23 ± 1.26	12.32	1.29	10.39 ± 1.23	11.84	2.87
	25.25	23.85 ± 3.52	14.76	−5.54	23.85 ± 3.21	13.46	−5.54
Brain	5.05	4.95 ± 0.72	14.55	−1.98	4.65 ± 0.61	13.12	−7.92
	10.10	9.43 ± 1.15	12.20	−6.63	8.89 ± 0.87	9.79	−11.98
	25.25	23.40 ± 2.89	12.35	−7.33	22.85 ± 2.34	10.24	−9.50
Stomach	8.10	8.89 ± 1.20	13.50	9.75	9.13 ± 1.32	14.46	12.72
	81.00	86.11 ± 8.14	9.45	6.31	87.58 ± 9.09	10.38	8.12
	162.00	154.67 ± 21.08	13.63	−4.52	165.48 ± 16.47	9.95	2.15
Uterus	5.05	4.80 ± 0.60	12.50	−4.95	4.61 ± 0.46	9.98	−8.71
	10.10	10.15 ± 1.47	14.48	0.50	9.68 ± 1.30	13.43	−4.16
	25.25	25.85 ± 3.22	12.46	2.38	25.33 ± 3.72	14.69	0.32
Ovary	8.10	9.10 ± 1.21	13.30	12.35	9.11 ± 1.22	13.39	12.47
	40.50	41.81 ± 6.20	14.83	3.23	44.33 ± 5.85	13.20	9.46
	81.00	87.39 ± 12.01	13.74	7.89	85.47 ± 11.40	13.34	5.52
Intestine	8.10	9.13 ± 1.27	13.91	12.72	8.71 ± 1.12	12.86	7.53
	40.50	38.26 ± 5.07	13.25	−5.53	40.21 ± 5.18	12.88	−0.72
	81.00	82.56 ± 8.73	10.57	1.93	78.73 ± 8.08	10.26	−2.80