Research Article
A Human Oral Fluid Assay for D- and L- Isomer Detection of Amphetamine and Methamphetamine Using Liquid-Liquid Extraction
Table 1
LC-MS/MS conditions for the three assays on a single oral fluid sample.
| | D- and L- |
| Scan type | MRM | Ion source | Turbo spray | Probe position | X = 5.00, Y = 5.2 | Polarity | Positive | Run duration | 11 min | Settling time (msec) | 0 | Pause time (msec) | 7.007 msec | Curtain gas | 35 | CAD gas | 4 | ISV (V) | 5000 | Temperature (°C) | 500 | Ion source gas 1 (GS 1) | 50 | Ion source gas 2 (GS 2) | 50 | Q1/Q3 resolution: | unit/unit | CEM (V) | 2600 | Inlet settings | | Analytical column | Supelco Astek Chirobiotic V 250 × 2.1 mm, 5 μm | Guard cartridge | None | Sample temperature | 15 ± 5.0°C | Column temperature | 30.0 ± 5.0°C | Mobile phase A | Water : acetic acid : ammonium hydroxide : Methanol 5 : 1 : 0.3 : 993.5 | Mobile phase B | N/A | Needle rinse | Water : acetic acid : ammonium hydroxide : Methanol 5 : 1 : 0.3 : 993.5 | Flow rate | 0.3 mL/min | Injection volume | 10 μL | Run time | 11 min |
|
|
Note. collision gas (CAD), ion source voltage (ISV), channel electron multiplier (CEM).
|