Research Article
Measurement of Pharmacokinetics and Tissue Distribution of Four Compounds from Nauclea officinalis in Rat Plasma and Tissues through HPLC-MS/MS
Table 2
Summary of standard curves, correlation coefficients, LLOQ, and linear ranges of four analytes in plasma and tissue samples.
| | Compounds | Matrix | Linear regression equation | R 2 | LLOQ (ng/mL) | Linear range (ng/mL) |
| | Chlorogenic acid | Plasma | y = 0.0288x + 0.00193 | 0.9994 | 2 | 2–2500 | | Heart | y = 0.0063x − 0.00771 | 0.9849 | 2 | 2–2500 | | Liver | y = 0.0267x − 0.0323 | 0.9980 | 2 | 2–2500 | | Spleen | y = 0.0392x + 0.0277 | 0.9886 | 2 | 2–2500 | | Lung | y = 0.0442x + 0.0239 | 0.9958 | 2 | 2–2500 | | Kidney | y = 0.0217x − 0.0302 | 0.9950 | 2 | 2–2500 | | Small intestine | y = 0.0334x − 0.0401 | 0.9964 | 2 | 2–2500 | | Stomach | y = 0.0402x − 0.0129 | 0.9952 | 2 | 2–2500 | | Brain | y = 0.0499x − 0.0562 | 0.9960 | 2 | 2–2500 |
| | Naucleactonin C | Plasma | y = 0.00535x + 0.0207 | 0.9964 | 5 | 5–2500 | | Heart | y = 0.00313x − 0.00116 | 0.9926 | 5 | 5–2500 | | Liver | y = 0.00395x − 0.0101 | 0.9964 | 5 | 5–2500 | | Spleen | y = 0.00511x − 0.00526 | 0.9950 | 5 | 5–2500 | | Lung | y = 0.00534x − 0.00263 | 0.9980 | 5 | 5–2500 | | Kidney | y = 0.00363x − 0.0098 | 0.9960 | 5 | 5–2500 | | Small intestine | y = 0.00224x − 0.00527 | 0.9968 | 5 | 5–2500 | | Stomach | y = 0.00465x − 0.0138 | 0.9972 | 5 | 5–2500 | | Brain | y = 0.00552x − 0.0163 | 0.9932 | 5 | 5–2500 |
| | Khaephuoside A | Plasma | y = 0.00316x + 0.0153 | 0.9954 | 5 | 5–2000 | | Heart | y = 0.00673x − 0.005 | 0.9946 | 5 | 5–2000 | | Liver | y = 0.0063x − 0.00502 | 0.9962 | 5 | 5–2000 | | Spleen | y = 0.011x − 0.0054 | 0.9980 | 5 | 5–2000 | | Lung | y = 0.0099x − 0.00166 | 0.9978 | 5 | 5–2000 | | Kidney | y = 0.00531x − 0.0047 | 0.9954 | 5 | 5–2000 | | Small intestine | y = 0.0058x − 0.0156 | 0.9906 | 5 | 5–2000 | | Stomach | y = 0.00745x − 0.00837 | 0.9920 | 5 | 5–2000 | | Brain | y = 0.0126x + 0.000377 | 0.9938 | 5 | 5–2000 |
| | 3,4-Dimethoxyphenyl-1-O-β-apiofuroseyl(1 ⟶ 2)-β-D-glucopyranoside | Plasma | y = 0.000845x + 0.00396 | 0.9984 | 25 | 25–2500 | | Heart | y = 0.000804x + 0.00159 | 0.9849 | 25 | 25–2500 | | Liver | y = 0.000873x − 0.00515 | 0.9968 | 25 | 25–2500 | | Spleen | y = 0.00134x + 0.00947 | 0.9894 | 25 | 25–2500 | | Lung | y = 0.00138x + 0.00626 | 0.9964 | 25 | 25–2500 | | Kidney | y = 0.000633x − 0.000538 | 0.9930 | 25 | 25–2500 | | Small intestine | y = 0.00109x + 0.000966 | 0.9962 | 25 | 25–2500 | | Stomach | y = 0.00119x + 0.00549 | 0.9964 | 25 | 25–2500 | | Brain | y = 0.00163x − 0.0149 | 0.9952 | 25 | 25–2500 |
|
|
Abbreviations. LLOQ, lower limit of quantification; R2, determination coefficients.
|
