Measurement of Pharmacokinetics and Tissue Distribution of Four Compounds from Nauclea officinalis in Rat Plasma and Tissues through HPLC-MS/MS

Table 3

Summary of intraday and interday precisions and accuracies of four analytes in plasma and tissue samples.

Compounds	Matrix	Concentration (ng/mL)	Intraday (n = 6)		Interday (n = 18)
			Accuracy	RSD (%)	Accuracy	RSD (%)
Chlorogenic acid	Plasma	2	104.02 ± 11.91	11.45	100.64 ± 11.67	11.60
		4	97.72 ± 9.23	9.45	96.09 ± 7.08	7.36
		100	104.42 ± 6.23	5.97	104.29 ± 10.6	10.16
		2400	101.22 ± 3.20	3.16	96.59 ± 8.11	8.40
	Heart	2	101.90 ± 12.14	11.92	109.34 ± 11.74	10.74
		4	99.35 ± 3.89	3.91	93.67 ± 6.13	6.54
		100	96.57 ± 4.81	4.98	94.46 ± 7.80	8.26
		2400	109.00 ± 5.90	5.41	102.88 ± 6.85	6.66
	Liver	2	101.65 ± 2.82	2.78	100.88 ± 5.38	5.33
		4	101.45 ± 2.98	2.93	98.59 ± 6.72	6.82
		100	100.30 ± 2.89	2.88	97.78 ± 4.89	5.00
		2400	95.07 ± 5.74	6.04	97.11 ± 5.58	5.75
	Spleen	2	96.40 ± 7.63	7.92	102.14 ± 8.90	8.71
		4	95.40 ± 4.20	4.40	101.28 ± 9.61	9.49
		100	102.40 ± 2.00	1.95	111.52 ± 7.00	6.27
		2400	94.93 ± 4.81	5.07	108.26 ± 10.41	9.62
	Lung	2	101.53 ± 6.40	6.30	101.22 ± 7.45	7.36
		4	99.75 ± 6.09	6.10	93.33 ± 11.17	11.97
		100	108.33 ± 5.47	5.04	111.33 ± 6.49	5.83
		2400	100.60 ± 3.35	3.33	101.63 ± 5.07	4.99
	Kidney	2	98.12 ± 5.36	5.47	96.23 ± 5.33	5.54
		4	100.13 ± 2.17	2.17	92.09 ± 7.21	7.83
		100	99.37 ± 5.20	5.23	96.10 ± 6.36	6.62
		2400	104.47 ± 5.79	5.54	99.47 ± 6.06	6.10
	Small intestine	2	93.27 ± 4.13	4.43	96.03 ± 7.86	8.19
		4	86.48 ± 5.09	5.89	93.40 ± 10.75	11.51
		100	89.75 ± 7.96	8.87	96.61 ± 10.06	10.41
		2400	92.70 ± 6.63	7.16	93.01 ± 5.27	5.66
	Stomach	2	105.87 ± 7.87	7.43	107.84 ± 7.45	6.90
		4	93.17 ± 7.02	7.53	98.76 ± 6.44	6.52
		100	106.00 ± 3.95	3.73	106.35 ± 4.46	4.19
		2400	100.63 ± 4.18	4.15	99.41 ± 4.81	4.84
	Brain	2	109.27 ± 6.74	6.17	104.39 ± 9.47	9.07
		4	101.00 ± 3.81	3.77	101.29 ± 5.42	5.35
		100	106.00 ± 4.94	4.66	106.77 ± 7.16	6.70
		2400	101.27 ± 2.25	2.22	105.62 ± 7.04	6.67
Naucleactonin C	Plasma	5	95.62 ± 12.10	12.65	98.24 ± 10.30	10.48
		8	100.53 ± 11.78	11.71	96.43 ± 12.16	12.61
		140	99.95 ± 6.49	6.50	95.76 ± 7.18	7.50
		2400	97.05 ± 4.80	4.95	95.50 ± 6.23	6.52
	Heart	5	111.27 ± 7.32	6.58	107.46 ± 10.58	9.85
		8	99.28 ± 5.48	5.52	101.15 ± 11.46	11.33
		140	93.45 ± 4.34	4.65	93.19 ± 5.33	5.72
		2400	105.92 ± 9.54	9.01	104.46 ± 6.66	6.38
	Liver	5	100.90 ± 5.40	5.36	108.30 ± 7.82	7.22
		8	99.47 ± 2.82	2.84	100.52 ± 5.00	4.98
		140	88.60 ± 2.60	2.93	93.53 ± 6.33	6.77
		2400	102.50 ± 1.52	1.48	101.42 ± 2.46	2.42
	Spleen	5	111.00 ± 6.99	6.29	104.42 ± 9.31	8.91
		8	98.90 ± 3.55	3.59	100.74 ± 6.77	6.72
		140	96.07 ± 3.95	4.11	93.72 ± 5.30	5.66
		2400	100.02 ± 3.39	3.39	103.13 ± 5.06	4.91
	Lung	5	113.83 ± 4.07	3.58	113.44 ± 5.37	4.73
		8	103.28 ± 10.46	10.13	102.03 ± 10.16	9.96
		140	104.17 ± 3.76	3.61	103.83 ± 4.05	3.90
		2400	107.83 ± 4.07	3.77	107.06 ± 5.02	4.69
	Kidney	5	100.70 ± 4.28	4.25	103.81 ± 6.61	6.36
		8	98.38 ± 5.31	5.40	99.18 ± 4.36	4.39
		140	95.37 ± 4.19	4.39	100.44 ± 7.07	7.04
		2400	106.80 ± 9.74	9.12	104.87 ± 6.46	6.16
	Small intestine	5	109.98 ± 10.97	9.97	105.41 ± 10.19	9.67
		8	99.30 ± 8.94	9.01	100.44 ± 9.18	9.14
		140	88.73 ± 8.96	10.10	86.31 ± 7.66	8.88
		2400	103.30 ± 6.82	6.60	98.26 ± 7.60	7.73
	Stomach	5	109.83 ± 7.05	6.42	102.67 ± 10.09	9.83
		8	101.58 ± 2.96	2.92	95.73 ± 8.07	8.43
		140	94.88 ± 2.74	2.88	92.09 ± 5.17	5.62
		2400	113.17 ± 4.88	4.31	108.96 ± 7.21	6.62
	Brain	5	105.75 ± 10.81	10.22	105.82 ± 8.77	8.29
		8	104.78 ± 7.46	7.12	100.57 ± 8.11	8.06
		140	101.22 ± 4.59	4.53	98.93 ± 5.73	5.80
		2400	113.83 ± 3.31	2.91	111.39 ± 5.54	4.98
Khaephuoside A	Plasma	5	101.63 ± 9.93	9.77	105.66 ± 10.50	9.94
		8	101.02 ± 9.64	9.55	101.78 ± 7.85	7.71
		120	112.33 ± 4.76	4.24	106.16 ± 6.11	5.76
		1800	104.08 ± 4.39	4.21	102.52 ± 4.41	4.31
	Heart	5	113.50 ± 9.18	8.09	103.33 ± 10.92	10.57
		8	100.82 ± 6.19	6.14	98.68 ± 7.06	7.16
		120	96.73 ± 2.92	3.02	89.72 ± 8.01	8.93
		1800	109.12 ± 9.45	8.66	102.43 ± 8.13	7.94
	Liver	5	99.97 ± 7.06	7.07	101.51 ± 8.49	8.36
		8	104.10 ± 5.07	4.87	101.81 ± 4.02	3.95
		120	99.33 ± 0.62	0.62	100.43 ± 4.55	4.53
		1800	108.00 ± 6.10	5.65	103.94 ± 5.60	5.39
	Spleen	5	98.73 ± 11.21	11.35	103.62 ± 9.47	9.14
		8	100.98 ± 6.25	6.19	103.08 ± 6.75	6.55
		120	99.35 ± 2.52	2.54	109.89 ± 8.84	8.04
		1800	109.00 ± 4.60	4.22	113.06 ± 5.36	4.74
	Lung	5	108.17 ± 10.19	9.42	109.93 ± 7.6	6.92
		8	109.90 ± 11.25	10.24	106.91 ± 9.95	9.31
		120	90.32 ± 6.12	6.78	95.54 ± 6.53	6.83
		1800	100.73 ± 2.02	2.00	103.65 ± 6.91	6.66
	Kidney	5	106.82 ± 7.91	7.40	109.94 ± 6.29	5.72
		8	113.67 ± 2.94	2.59	111.40 ± 6.12	5.49
		120	105.30 ± 6.14	5.83	107.78 ± 6.91	6.41
		1800	105.02 ± 9.81	9.34	108.84 ± 7.60	6.99
	Small intestine	5	106.50 ± 8.53	8.01	104.08 ± 10.11	9.71
		8	99.98 ± 9.43	9.13	101.89 ± 11.58	11.36
		120	89.90 ± 6.84	7.61	93.74 ± 7.80	8.32
		1800	104.45 ± 6.22	5.96	105.44 ± 5.15	4.89
	Stomach	5	96.63 ± 9.15	9.47	95.98 ± 10.26	10.69
		8	90.92 ± 7.96	8.76	97.66 ± 8.61	8.82
		120	104.00 ± 6.13	5.90	103.24 ± 5.31	5.15
		1800	109.83 ± 4.83	4.40	110.50 ± 4.54	4.11
	Brain	5	108.90 ± 9.74	8.95	107.11 ± 7.94	7.41
		8	108.55 ± 9.90	9.12	107.23 ± 8.01	7.47
		120	113.17 ± 6.71	5.93	110.13 ± 6.09	5.53
		1800	100.50 ± 3.99	3.97	108.44 ± 7.63	7.03
3,4-Dimethoxyphenyl-1-O-β-apiofuroseyl(1 ⟶ 2)-β-D-glucopyranoside	Plasma	25	109.00 ± 4.05	3.72	101.34 ± 9.10	8.98
		50	105.82 ± 10.95	10.35	102.61 ± 7.89	7.69
		350	109.83 ± 5.23	4.76	104.49 ± 8.60	8.23
		2400	96.82 ± 2.76	2.85	100.71 ± 7.50	7.45
	Heart	25	99.12 ± 5.06	5.10	96.73 ± 8.08	8.35
		50	96.45 ± 6.75	7.00	98.20 ± 8.28	8.43
		350	89.15 ± 4.83	5.42	90.19 ± 6.82	7.56
		2400	99.80 ± 10.89	10.91	99.33 ± 7.66	7.71
	Liver	25	105.07 ± 6.62	6.30	103.78 ± 6.39	6.16
		50	108.48 ± 7.39	6.82	108.22 ± 7.38	6.82
		350	112.15 ± 6.89	6.14	113.99 ± 4.83	4.24
		2400	98.28 ± 3.67	3.73	100.28 ± 3.24	3.23
	Spleen	25	102.03 ± 10.68	10.47	104.78 ± 8.63	8.23
		50	99.90 ± 520	5.21	103.82 ± 7.77	7.48
		350	97.70 ± 4.12	4.21	109.12 ± 9.07	8.31
		2400	100.65 ± 5.46	5.43	110.22 ± 8.94	8.11
	Lung	25	96.17 ± 7.59	7.89	99.80 ± 10.99	11.01
		50	106.78 ± 7.08	6.63	107.49 ± 8.16	7.59
		350	96.42 ± 4.49	4.66	100.05 ± 5.50	5.49
		2400	99.67 ± 6.29	6.31	99.32 ± 5.68	5.72
	Kidney	25	97.02 ± 9.75	10.05	96.23 ± 9.38	9.74
		50	108.00 ± 5.66	5.24	106.69 ± 7.00	6.56
		350	99.63 ± 5.83	5.85	100.46 ± 6.78	6.75
		2400	96.50 ± 7.41	7.68	97.74 ± 6.31	6.46
	Small intestine	25	98.25 ± 8.92	9.08	97.96 ± 7.73	7.90
		50	101.58 ± 6.41	6.31	101.89 ± 7.13	7.00
		350	89.58 ± 4.27	4.76	98.17 ± 8.39	8.55
		2400	100.18 ± 4.77	4.76	101.32 ± 4.83	4.77
	Stomach	25	103.63 ± 5.21	5.02	99.07 ± 9.64	9.73
		50	101.90 ± 9.32	9.14	97.96 ± 9.33	9.52
		350	114.17 ± 3.19	2.79	109.34 ± 6.14	5.62
		2400	100.62 ± 3.42	3.40	99.42 ± 4.66	4.69
	Brain	25	106.95 ± 12.13	11.34	99.13 ± 11.26	11.36
		50	105.78 ± 7.63	7.22	107.10 ± 7.86	7.34
		350	110.00 ± 9.01	8.19	112.56 ± 6.93	6.16
		2400	99.42 ± 2.90	2.91	101.13 ± 5.44	5.38