Research Article
Measurement of Pharmacokinetics and Tissue Distribution of Four Compounds from Nauclea officinalis in Rat Plasma and Tissues through HPLC-MS/MS
Table 3
Summary of intraday and interday precisions and accuracies of four analytes in plasma and tissue samples.
| Compounds | Matrix | Concentration (ng/mL) | Intraday (n = 6) | Interday (n = 18) | Accuracy | RSD (%) | Accuracy | RSD (%) |
| Chlorogenic acid | Plasma | 2 | 104.02 ± 11.91 | 11.45 | 100.64 ± 11.67 | 11.60 | 4 | 97.72 ± 9.23 | 9.45 | 96.09 ± 7.08 | 7.36 | 100 | 104.42 ± 6.23 | 5.97 | 104.29 ± 10.6 | 10.16 | 2400 | 101.22 ± 3.20 | 3.16 | 96.59 ± 8.11 | 8.40 | Heart | 2 | 101.90 ± 12.14 | 11.92 | 109.34 ± 11.74 | 10.74 | 4 | 99.35 ± 3.89 | 3.91 | 93.67 ± 6.13 | 6.54 | 100 | 96.57 ± 4.81 | 4.98 | 94.46 ± 7.80 | 8.26 | 2400 | 109.00 ± 5.90 | 5.41 | 102.88 ± 6.85 | 6.66 | Liver | 2 | 101.65 ± 2.82 | 2.78 | 100.88 ± 5.38 | 5.33 | 4 | 101.45 ± 2.98 | 2.93 | 98.59 ± 6.72 | 6.82 | 100 | 100.30 ± 2.89 | 2.88 | 97.78 ± 4.89 | 5.00 | 2400 | 95.07 ± 5.74 | 6.04 | 97.11 ± 5.58 | 5.75 | Spleen | 2 | 96.40 ± 7.63 | 7.92 | 102.14 ± 8.90 | 8.71 | 4 | 95.40 ± 4.20 | 4.40 | 101.28 ± 9.61 | 9.49 | 100 | 102.40 ± 2.00 | 1.95 | 111.52 ± 7.00 | 6.27 | 2400 | 94.93 ± 4.81 | 5.07 | 108.26 ± 10.41 | 9.62 | Lung | 2 | 101.53 ± 6.40 | 6.30 | 101.22 ± 7.45 | 7.36 | 4 | 99.75 ± 6.09 | 6.10 | 93.33 ± 11.17 | 11.97 | 100 | 108.33 ± 5.47 | 5.04 | 111.33 ± 6.49 | 5.83 | 2400 | 100.60 ± 3.35 | 3.33 | 101.63 ± 5.07 | 4.99 | Kidney | 2 | 98.12 ± 5.36 | 5.47 | 96.23 ± 5.33 | 5.54 | 4 | 100.13 ± 2.17 | 2.17 | 92.09 ± 7.21 | 7.83 | 100 | 99.37 ± 5.20 | 5.23 | 96.10 ± 6.36 | 6.62 | 2400 | 104.47 ± 5.79 | 5.54 | 99.47 ± 6.06 | 6.10 | Small intestine | 2 | 93.27 ± 4.13 | 4.43 | 96.03 ± 7.86 | 8.19 | 4 | 86.48 ± 5.09 | 5.89 | 93.40 ± 10.75 | 11.51 | 100 | 89.75 ± 7.96 | 8.87 | 96.61 ± 10.06 | 10.41 | 2400 | 92.70 ± 6.63 | 7.16 | 93.01 ± 5.27 | 5.66 | Stomach | 2 | 105.87 ± 7.87 | 7.43 | 107.84 ± 7.45 | 6.90 | 4 | 93.17 ± 7.02 | 7.53 | 98.76 ± 6.44 | 6.52 | 100 | 106.00 ± 3.95 | 3.73 | 106.35 ± 4.46 | 4.19 | 2400 | 100.63 ± 4.18 | 4.15 | 99.41 ± 4.81 | 4.84 | Brain | 2 | 109.27 ± 6.74 | 6.17 | 104.39 ± 9.47 | 9.07 | 4 | 101.00 ± 3.81 | 3.77 | 101.29 ± 5.42 | 5.35 | 100 | 106.00 ± 4.94 | 4.66 | 106.77 ± 7.16 | 6.70 | 2400 | 101.27 ± 2.25 | 2.22 | 105.62 ± 7.04 | 6.67 |
| Naucleactonin C | Plasma | 5 | 95.62 ± 12.10 | 12.65 | 98.24 ± 10.30 | 10.48 | 8 | 100.53 ± 11.78 | 11.71 | 96.43 ± 12.16 | 12.61 | 140 | 99.95 ± 6.49 | 6.50 | 95.76 ± 7.18 | 7.50 | 2400 | 97.05 ± 4.80 | 4.95 | 95.50 ± 6.23 | 6.52 | Heart | 5 | 111.27 ± 7.32 | 6.58 | 107.46 ± 10.58 | 9.85 | 8 | 99.28 ± 5.48 | 5.52 | 101.15 ± 11.46 | 11.33 | 140 | 93.45 ± 4.34 | 4.65 | 93.19 ± 5.33 | 5.72 | 2400 | 105.92 ± 9.54 | 9.01 | 104.46 ± 6.66 | 6.38 | Liver | 5 | 100.90 ± 5.40 | 5.36 | 108.30 ± 7.82 | 7.22 | 8 | 99.47 ± 2.82 | 2.84 | 100.52 ± 5.00 | 4.98 | 140 | 88.60 ± 2.60 | 2.93 | 93.53 ± 6.33 | 6.77 | 2400 | 102.50 ± 1.52 | 1.48 | 101.42 ± 2.46 | 2.42 | Spleen | 5 | 111.00 ± 6.99 | 6.29 | 104.42 ± 9.31 | 8.91 | 8 | 98.90 ± 3.55 | 3.59 | 100.74 ± 6.77 | 6.72 | 140 | 96.07 ± 3.95 | 4.11 | 93.72 ± 5.30 | 5.66 | 2400 | 100.02 ± 3.39 | 3.39 | 103.13 ± 5.06 | 4.91 | Lung | 5 | 113.83 ± 4.07 | 3.58 | 113.44 ± 5.37 | 4.73 | 8 | 103.28 ± 10.46 | 10.13 | 102.03 ± 10.16 | 9.96 | 140 | 104.17 ± 3.76 | 3.61 | 103.83 ± 4.05 | 3.90 | 2400 | 107.83 ± 4.07 | 3.77 | 107.06 ± 5.02 | 4.69 | Kidney | 5 | 100.70 ± 4.28 | 4.25 | 103.81 ± 6.61 | 6.36 | 8 | 98.38 ± 5.31 | 5.40 | 99.18 ± 4.36 | 4.39 | 140 | 95.37 ± 4.19 | 4.39 | 100.44 ± 7.07 | 7.04 | 2400 | 106.80 ± 9.74 | 9.12 | 104.87 ± 6.46 | 6.16 | Small intestine | 5 | 109.98 ± 10.97 | 9.97 | 105.41 ± 10.19 | 9.67 | 8 | 99.30 ± 8.94 | 9.01 | 100.44 ± 9.18 | 9.14 | 140 | 88.73 ± 8.96 | 10.10 | 86.31 ± 7.66 | 8.88 | 2400 | 103.30 ± 6.82 | 6.60 | 98.26 ± 7.60 | 7.73 | Stomach | 5 | 109.83 ± 7.05 | 6.42 | 102.67 ± 10.09 | 9.83 | 8 | 101.58 ± 2.96 | 2.92 | 95.73 ± 8.07 | 8.43 | 140 | 94.88 ± 2.74 | 2.88 | 92.09 ± 5.17 | 5.62 | 2400 | 113.17 ± 4.88 | 4.31 | 108.96 ± 7.21 | 6.62 | Brain | 5 | 105.75 ± 10.81 | 10.22 | 105.82 ± 8.77 | 8.29 | 8 | 104.78 ± 7.46 | 7.12 | 100.57 ± 8.11 | 8.06 | 140 | 101.22 ± 4.59 | 4.53 | 98.93 ± 5.73 | 5.80 | 2400 | 113.83 ± 3.31 | 2.91 | 111.39 ± 5.54 | 4.98 |
| Khaephuoside A | Plasma | 5 | 101.63 ± 9.93 | 9.77 | 105.66 ± 10.50 | 9.94 | 8 | 101.02 ± 9.64 | 9.55 | 101.78 ± 7.85 | 7.71 | 120 | 112.33 ± 4.76 | 4.24 | 106.16 ± 6.11 | 5.76 | 1800 | 104.08 ± 4.39 | 4.21 | 102.52 ± 4.41 | 4.31 | Heart | 5 | 113.50 ± 9.18 | 8.09 | 103.33 ± 10.92 | 10.57 | 8 | 100.82 ± 6.19 | 6.14 | 98.68 ± 7.06 | 7.16 | 120 | 96.73 ± 2.92 | 3.02 | 89.72 ± 8.01 | 8.93 | 1800 | 109.12 ± 9.45 | 8.66 | 102.43 ± 8.13 | 7.94 | Liver | 5 | 99.97 ± 7.06 | 7.07 | 101.51 ± 8.49 | 8.36 | 8 | 104.10 ± 5.07 | 4.87 | 101.81 ± 4.02 | 3.95 | 120 | 99.33 ± 0.62 | 0.62 | 100.43 ± 4.55 | 4.53 | 1800 | 108.00 ± 6.10 | 5.65 | 103.94 ± 5.60 | 5.39 | Spleen | 5 | 98.73 ± 11.21 | 11.35 | 103.62 ± 9.47 | 9.14 | 8 | 100.98 ± 6.25 | 6.19 | 103.08 ± 6.75 | 6.55 | 120 | 99.35 ± 2.52 | 2.54 | 109.89 ± 8.84 | 8.04 | 1800 | 109.00 ± 4.60 | 4.22 | 113.06 ± 5.36 | 4.74 | Lung | 5 | 108.17 ± 10.19 | 9.42 | 109.93 ± 7.6 | 6.92 | 8 | 109.90 ± 11.25 | 10.24 | 106.91 ± 9.95 | 9.31 | 120 | 90.32 ± 6.12 | 6.78 | 95.54 ± 6.53 | 6.83 | 1800 | 100.73 ± 2.02 | 2.00 | 103.65 ± 6.91 | 6.66 | Kidney | 5 | 106.82 ± 7.91 | 7.40 | 109.94 ± 6.29 | 5.72 | 8 | 113.67 ± 2.94 | 2.59 | 111.40 ± 6.12 | 5.49 | 120 | 105.30 ± 6.14 | 5.83 | 107.78 ± 6.91 | 6.41 | 1800 | 105.02 ± 9.81 | 9.34 | 108.84 ± 7.60 | 6.99 | Small intestine | 5 | 106.50 ± 8.53 | 8.01 | 104.08 ± 10.11 | 9.71 | 8 | 99.98 ± 9.43 | 9.13 | 101.89 ± 11.58 | 11.36 | 120 | 89.90 ± 6.84 | 7.61 | 93.74 ± 7.80 | 8.32 | 1800 | 104.45 ± 6.22 | 5.96 | 105.44 ± 5.15 | 4.89 | Stomach | 5 | 96.63 ± 9.15 | 9.47 | 95.98 ± 10.26 | 10.69 | 8 | 90.92 ± 7.96 | 8.76 | 97.66 ± 8.61 | 8.82 | 120 | 104.00 ± 6.13 | 5.90 | 103.24 ± 5.31 | 5.15 | 1800 | 109.83 ± 4.83 | 4.40 | 110.50 ± 4.54 | 4.11 | Brain | 5 | 108.90 ± 9.74 | 8.95 | 107.11 ± 7.94 | 7.41 | 8 | 108.55 ± 9.90 | 9.12 | 107.23 ± 8.01 | 7.47 | 120 | 113.17 ± 6.71 | 5.93 | 110.13 ± 6.09 | 5.53 | 1800 | 100.50 ± 3.99 | 3.97 | 108.44 ± 7.63 | 7.03 |
| 3,4-Dimethoxyphenyl-1-O-β-apiofuroseyl(1 ⟶ 2)-β-D-glucopyranoside | Plasma | 25 | 109.00 ± 4.05 | 3.72 | 101.34 ± 9.10 | 8.98 | 50 | 105.82 ± 10.95 | 10.35 | 102.61 ± 7.89 | 7.69 | 350 | 109.83 ± 5.23 | 4.76 | 104.49 ± 8.60 | 8.23 | 2400 | 96.82 ± 2.76 | 2.85 | 100.71 ± 7.50 | 7.45 | Heart | 25 | 99.12 ± 5.06 | 5.10 | 96.73 ± 8.08 | 8.35 | 50 | 96.45 ± 6.75 | 7.00 | 98.20 ± 8.28 | 8.43 | 350 | 89.15 ± 4.83 | 5.42 | 90.19 ± 6.82 | 7.56 | 2400 | 99.80 ± 10.89 | 10.91 | 99.33 ± 7.66 | 7.71 | Liver | 25 | 105.07 ± 6.62 | 6.30 | 103.78 ± 6.39 | 6.16 | 50 | 108.48 ± 7.39 | 6.82 | 108.22 ± 7.38 | 6.82 | 350 | 112.15 ± 6.89 | 6.14 | 113.99 ± 4.83 | 4.24 | 2400 | 98.28 ± 3.67 | 3.73 | 100.28 ± 3.24 | 3.23 | Spleen | 25 | 102.03 ± 10.68 | 10.47 | 104.78 ± 8.63 | 8.23 | 50 | 99.90 ± 520 | 5.21 | 103.82 ± 7.77 | 7.48 | 350 | 97.70 ± 4.12 | 4.21 | 109.12 ± 9.07 | 8.31 | 2400 | 100.65 ± 5.46 | 5.43 | 110.22 ± 8.94 | 8.11 | Lung | 25 | 96.17 ± 7.59 | 7.89 | 99.80 ± 10.99 | 11.01 | 50 | 106.78 ± 7.08 | 6.63 | 107.49 ± 8.16 | 7.59 | 350 | 96.42 ± 4.49 | 4.66 | 100.05 ± 5.50 | 5.49 | 2400 | 99.67 ± 6.29 | 6.31 | 99.32 ± 5.68 | 5.72 | Kidney | 25 | 97.02 ± 9.75 | 10.05 | 96.23 ± 9.38 | 9.74 | 50 | 108.00 ± 5.66 | 5.24 | 106.69 ± 7.00 | 6.56 | 350 | 99.63 ± 5.83 | 5.85 | 100.46 ± 6.78 | 6.75 | 2400 | 96.50 ± 7.41 | 7.68 | 97.74 ± 6.31 | 6.46 | Small intestine | 25 | 98.25 ± 8.92 | 9.08 | 97.96 ± 7.73 | 7.90 | 50 | 101.58 ± 6.41 | 6.31 | 101.89 ± 7.13 | 7.00 | 350 | 89.58 ± 4.27 | 4.76 | 98.17 ± 8.39 | 8.55 | 2400 | 100.18 ± 4.77 | 4.76 | 101.32 ± 4.83 | 4.77 | Stomach | 25 | 103.63 ± 5.21 | 5.02 | 99.07 ± 9.64 | 9.73 | 50 | 101.90 ± 9.32 | 9.14 | 97.96 ± 9.33 | 9.52 | 350 | 114.17 ± 3.19 | 2.79 | 109.34 ± 6.14 | 5.62 | 2400 | 100.62 ± 3.42 | 3.40 | 99.42 ± 4.66 | 4.69 | Brain | 25 | 106.95 ± 12.13 | 11.34 | 99.13 ± 11.26 | 11.36 | 50 | 105.78 ± 7.63 | 7.22 | 107.10 ± 7.86 | 7.34 | 350 | 110.00 ± 9.01 | 8.19 | 112.56 ± 6.93 | 6.16 | 2400 | 99.42 ± 2.90 | 2.91 | 101.13 ± 5.44 | 5.38 |
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