Measurement of Pharmacokinetics and Tissue Distribution of Four Compounds from Nauclea officinalis in Rat Plasma and Tissues through HPLC-MS/MS

Table 5

Summary of stability of four analytes in plasma and tissue samples (n = 6).

Compounds	Matrix	Concentration (ng/mL)	Ambient temperature for 4 h		Autosampler at 4°C for 8 h		Three freeze-thraw cycles		−80°C for 30 days
			Accuracy	RSD (%)	Accuracy	RSD (%)	Accuracy	RSD (%)	Accuracy	RSD (%)
Chlorogenic acid	Plasma	2	101.68 ± 8.39	8.25	98.25 ± 7.83	7.97	101.23 ± 12.44	12.29	93.35 ± 10.78	11.55
		4	86.78 ± 6.16	7.09	97.90 ± 11.88	12.14	95.02 ± 5.39	5.67	98.92 ± 12.32	12.46
		100	112.33 ± 3.72	3.31	111.33 ± 4.37	3.92	100.68 ± 6.22	6.18	113.17 ± 4.96	4.38
		2400	98.40 ± 5.56	5.65	99.47 ± 7.59	7.63	91.98 ± 4.75	5.17	112.67 ± 7.58	6.73
	Heart	2	102.73 ± 12.98	12.64	101.70 ± 10.91	10.72	91.72 ± 6.33	6.90	88.98 ± 5.73	6.43
		4	96.88 ± 11.06	11.42	99.50 ± 6.21	6.24	99.03 ± 10.17	10.27	102.27 ± 10.30	10.07
		100	112.83 ± 9.11	8.07	111.93 ± 11.77	10.52	111.00 ± 7.72	6.96	112.62 ± 8.15	7.24
		2400	108.60 ± 8.41	7.75	101.43 ± 10.34	10.20	110.55 ± 8.11	7.33	104.85 ± 10.69	10.19
	Liver	2	88.47 ± 6.93	7.83	87.82 ± 6.45	7.35	88.47 ± 7.22	8.16	87.43 ± 7.74	8.86
		4	86.27 ± 7.32	8.48	87.33 ± 5.74	6.57	86.00 ± 6.23	7.24	87.30 ± 4.35	4.98
		100	99.82 ± 2.89	2.89	106.33 ± 2.88	2.70	102.27 ± 4.97	4.86	87.13 ± 5.77	6.62
		2400	104.17 ± 2.14	2.05	100.92 ± 3.08	3.05	99.98 ± 1.86	1.86	87.02 ± 5.24	6.03
	Spleen	2	97.62 ± 8.11	8.30	109.83 ± 9.95	9.06	105.55 ± 7.56	7.17	109.70 ± 13.05	11.90
		4	105.82 ± 9.91	9.37	100.15 ± 9.69	9.67	103.68 ± 9.71	9.36	98.88 ± 4.16	4.20
		100	112.00 ± 7.82	6.98	111.12 ± 7.31	6.58	106.82 ± 6.88	6.44	111.17 ± 7.17	6.45
		2400	101.52 ± 7.73	7.61	104.00 ± 7.45	7.17	106.67 ± 5.43	5.09	104.73 ± 7.04	6.72
	Lung	2	103.27 ± 8.01	7.75	111.67 ± 5.43	4.86	112.93 ± 9.57	8.48	113.77 ± 9.20	8.08
		4	101.83 ± 6.41	6.30	100.55 ± 8.58	8.53	100.07 ± 10.39	10.38	98.77 ± 6.15	6.23
		100	111.50 ± 5.47	4.90	110.17 ± 10.93	9.92	110.17 ± 7.14	6.48	110.03 ± 9.33	8.46
		2400	100.67 ± 10.17	10.11	98.78 ± 7.01	7.10	104.88 ± 8.23	7.85	110.17 ± 4.54	4.12
	Kidney	2	110.07 ± 7.34	6.67	88.18 ± 4.98	5.64	97.22 ± 7.67	7.89	89.87 ± 9.23	10.27
		4	87.63 ± 7.76	8.86	88.40 ± 6.19	7.00	87.60 ± 9.26	10.58	89.60 ± 9.05	10.10
		100	112.00 ± 7.01	6.26	111.92 ± 8.86	7.91	111.17 ± 7.44	6.69	103.22 ± 11.32	10.97
		2400	100.55 ± 5.11	5.09	98.10 ± 4.87	4.97	100.45 ± 3.96	3.94	101.23 ± 4.69	4.63
	Small intestine	2	105.00 ± 8.20	7.81	102.63 ± 3.52	3.43	103.90 ± 4.49	4.32	101.42 ± 7.82	7.71
		4	91.73 ± 4.49	4.89	99.47 ± 5.10	5.13	99.78 ± 7.70	7.72	106.70 ± 5.43	5.09
		100	95.90 ± 12.31	12.84	108.67 ± 12.04	11.08	100.93 ± 12.33	12.22	99.07 ± 12.41	12.52
		2400	112.00 ± 5.37	4.79	111.67 ± 7.26	6.50	111.67 ± 6.12	5.48	110.83 ± 6.68	6.02
	Stomach	2	113.08 ± 11.06	9.78	111.67 ± 6.25	5.60	113.58 ± 8.22	7.24	109.08 ± 9.97	9.14
		4	102.70 ± 10.17	9.90	97.82 ± 9.05	9.25	98.87 ± 9.82	9.94	89.87 ± 7.04	7.84
		100	93.75 ± 8.65	9.22	94.82 ± 5.05	5.33	93.47 ± 6.76	7.23	107.33 ± 5.89	5.49
		2400	107.17 ± 8.68	8.10	96.60 ± 3.60	3.72	100.23 ± 4.63	4.62	99.73 ± 10.09	10.12
	Brain	2	110.33 ± 7.55	6.85	106.15 ± 12.36	11.65	105.65 ± 10.92	10.34	90.73 ± 10.25	11.30
		4	89.12 ± 9.00	10.10	87.57 ± 8.47	9.67	90.68 ± 10.51	11.59	105.32 ± 9.72	9.23
		100	91.93 ± 9.93	10.80	109.17 ± 7.44	6.82	108.13 ± 9.91	9.17	105.48 ± 6.92	6.56
		2400	102.93 ± 5.79	5.62	106.72 ± 9.83	9.21	96.57 ± 10.12	10.48	97.98 ± 9.56	9.75
Naucleactonin C	Plasma	5	101.95 ± 10.33	10.14	106.67 ± 10.88	10.20	91.87 ± 8.63	9.39	109.57 ± 12.66	11.55
		8	108.88 ± 1.87	1.72	105.01 ± 6.32	6.02	105.88 ± 1.87	1.77	113.33 ± 5.32	4.69
		140	87.67 ± 9.20	10.50	87.88 ± 4.14	4.71	111.88 ± 1.87	1.67	111.57 ± 9.60	8.60
		2400	88.93 ± 6.01	6.75	89.25 ± 7.58	8.49	89.17 ± 7.49	8.40	113.83 ± 7.14	6.27
	Heart	5	111.97 ± 11.63	10.39	100.78 ± 9.63	9.56	107.98 ± 9.45	8.75	107.42 ± 10.24	9.53
		8	110.50 ± 4.42	4.00	107.25 ± 9.25	8.62	109.08 ± 8.25	7.56	110.87 ± 9.04	8.15
		140	102.78 ± 6.89	6.71	104.88 ± 3.80	3.62	100.40 ± 9.13	9.09	98.28 ± 5.70	5.80
		2400	112.33 ± 10.41	9.26	111.50 ± 5.68	5.10	112.33 ± 5.72	5.09	110.50 ± 4.81	4.35
	Liver	5	101.78 ± 11.83	11.63	107.70 ± 5.36	4.98	98.32 ± 6.66	6.78	98.38 ± 12.07	12.27
		8	87.60 ± 5.90	6.73	86.47 ± 4.52	5.23	90.60 ± 4.91	5.42	86.00 ± 8.60	10.00
		140	88.57 ± 2.44	2.76	95.00 ± 4.15	4.37	96.28 ± 4.36	4.53	97.32 ± 7.17	7.36
		2400	98.93 ± 3.81	3.85	91.37 ± 2.51	2.75	89.35 ± 3.70	4.15	89.97 ± 7.32	8.14
	Spleen	5	110.33 ± 8.71	7.89	112.92 ± 8.72	7.72	110.67 ± 8.57	7.75	109.68 ± 12.57	11.46
		8	99.28 ± 13.31	13.41	93.42 ± 8.00	8.57	96.13 ± 11.98	12.46	101.90 ± 10.25	10.06
		140	93.13 ± 6.10	6.55	95.27 ± 7.08	7.43	89.18 ± 8.23	9.23	92.35 ± 2.70	2.92
		2400	103.33 ± 8.37	8.10	105.67 ± 8.50	8.05	107.73 ± 8.10	7.51	109.83 ± 4.96	4.51
	Lung	5	109.50 ± 11.47	10.48	100.40 ± 10.26	10.22	101.05 ± 5.33	5.28	95.98 ± 10.47	10.91
		8	94.73 ± 8.92	9.41	91.40 ± 9.71	10.63	101.90 ± 10.45	10.25	99.03 ± 10.49	10.59
		140	102.38 ± 4.12	4.03	94.45 ± 5.92	6.27	93.75 ± 8.53	9.09	92.58 ± 3.60	3.89
		2400	111.37 ± 9.27	8.33	111.85 ± 9.53	8.52	112.125 ± 9.66	8.61	111.67 ± 3.39	3.03
	Kidney	5	109.33 ± 7.55	6.91	106.08 ± 8.96	8.44	98.92 ± 9.91	10.02	97.18 ± 10.46	10.76
		8	99.10 ± 4.24	4.27	97.38 ± 7.31	7.51	91.10 ± 9.20	10.10	94.77 ± 7.62	8.04
		140	96.08 ± 7.36	7.66	95.43 ± 7.99	8.37	101.10 ± 4.98	4.92	100.25 ± 8.25	8.23
		2400	108.00 ± 5.62	5.20	103.87 ± 4.68	4.51	104.57 ± 6.09	5.82	102.30 ± 5.90	5.77
	Small intestine	5	111.90 ± 12.07	10.79	111.67 ± 8.14	7.29	111.17 ± 8.33	7.49	112.67 ± 8.78	7.79
		8	103.33 ± 3.39	3.28	100.03 ± 11.46	11.46	100.60 ± 10.24	10.18	104.25 ± 9.49	9.10
		140	88.60 ± 9.48	10.70	89.80 ± 8.30	9.24	87.88 ± 5.57	6.34	90.17 ± 7.26	8.05
		2400	107.48 ± 8.23	7.66	103.75 ± 6.68	6.44	108.43 ± 8.65	7.98	102.32 ± 8.65	8.45
	Stomach	5	111.00 ± 7.90	7.12	112.17 ± 7.25	6.46	113.12 ± 9.97	8.81	113.83 ± 2.99	2.63
		8	107.32 ± 12.96	12.08	98.37 ± 10.75	10.93	105.90 ± 9.13	8.62	103.55 ± 9.49	9.16
		140	88.15 ± 7.91	8.98	86.38 ± 8.38	9.70	87.12 ± 5.24	6.1	88.28 ± 6.31	7.15
		2400	100.53 ± 7.29	7.25	99.52 ± 5.85	5.88	104.90 ± 5.53	5.28	102.32 ± 8.22	8.03
	Brain	5	106.35 ± 11.82	11.11	109.83 ± 6.05	5.51	98.92 ± 10.65	10.77	104.10 ± 10.88	10.45
		8	88.87 ± 10.43	11.74	90.65 ± 6.37	7.03	89.95 ± 9.23	10.26	88.82 ± 10.57	11.90
		140	88.33 ± 6.74	7.63	87.92 ± 8.15	9.27	87.20 ± 4.98	5.71	87.93 ± 5.81	6.61
		2400	99.43 ± 6.38	6.41	112.67 ± 6.62	5.88	109.67 ± 6.44	5.87	109.00 ± 5.22	4.78
Khaephuoside A	Plasma	5	99.10 ± 9.25	9.33	90.97 ± 9.01	9.90	93.93 ± 11.26	11.98	101.18 ± 3.53	3.49
		8	91.38 ± 12.76	13.96	94.88 ± 12.31	12.97	90.67 ± 9.29	10.25	108.17 ± 9.20	8.50
		120	101.35 ± 4.33	4.28	101.32 ± 3.40	3.36	96.75 ± 4.55	4.70	107.30 ± 11.48	10.70
		1800	99.35 ± 6.04	6.08	101.93 ± 6.90	6.77	96.13 ± 4.03	4.19	112.67 ± 7.66	6.80
	Heart	5	107.60 ± 11.23	10.44	104.15 ± 9.92	9.52	110.67 ± 10.82	9.78	107.45 ± 9.35	8.70
		8	112.67 ± 7.23	6.42	111.83 ± 8.23	7.36	111.33 ± 5.92	5.32	101.00 ± 12.05	11.93
		120	111.67 ± 6.22	5.57	112.67 ± 5.89	5.23	108.58 ± 11.51	10.60	113.50 ± 7.20	6.35
		1800	110.83 ± 2.79	2.51	106.58 ± 8.77	8.22	113.67 ± 4.27	3.76	101.90 ± 12.30	12.07
	Liver	5	93.87 ± 9.31	9.92	106.17 ± 4.88	4.59	95.62 ± 9.82	10.27	100.87 ± 10.27	10.19
		8	108.95 ± 10.77	9.88	101.97 ± 7.93	7.78	105.20 ± 10.21	9.70	91.12 ± 10.79	11.84
		120	107.67 ± 6.22	5.78	111.50 ± 7.26	6.51	101.62 ± 9.62	9.47	104.23 ± 8.68	8.33
		1800	108.83 ± 2.32	2.13	109.83 ± 5.38	4.90	103.20 ± 7.52	7.29	108.93 ± 9.52	8.74
	Spleen	5	110.30 ± 12.31	11.16	112.17 ± 7.94	7.07	111.00 ± 10.26	9.24	111.50 ± 8.41	7.54
		8	112.17 ± 5.64	5.02	111.67 ± 6.19	5.54	111.38 ± 7.36	6.58	110.67 ± 7.17	6.48
		120	107.85 ± 7.83	7.26	105.45 ± 7.95	7.54	98.83 ± 7.51	7.60	105.42 ± 10.79	10.24
		1800	109.77 ± 8.61	7.85	111.17 ± 8.18	7.36	113.17 ± 3.31	2.93	111.83 ± 7.57	6.77
	Lung	5	113.33 ± 8.71	7.69	106.65 ± 12.43	11.65	98.87 ± 9.02	9.12	102.98 ± 9.50	9.23
		8	105.32 ± 7.81	7.41	103.18 ± 10.97	10.63	105.03 ± 9.86	9.38	104.00 ± 11.73	11.28
		120	101.27 ± 3.53	3.49	99.07 ± 11.01	11.11	102.02 ± 6.36	6.23	110.83 ± 5.19	4.69
		1800	110.83 ± 8.23	7.43	112.80 ± 7.46	6.61	110.00 ± 8.88	8.07	112.50 ± 6.80	6.05
	Kidney	5	96.42 ± 6.24	6.47	99.65 ± 10.53	10.57	101.62 ± 11.37	11.19	112.78 ± 11.43	10.13
		8	113.17 ± 6.46	5.71	110.83 ± 7.88	7.11	113.00 ± 6.99	6.18	113.17 ± 7.19	6.36
		120	111.67 ± 5.32	4.76	111.83 ± 5.31	4.75	112.00 ± 5.93	5.30	112.00 ± 5.62	5.02
		1800	102.03 ± 4.44	4.35	97.37 ± 6.84	7.02	100.50 ± 5.53	5.50	101.12 ± 6.56	6.48
	Small intestine	5	110.02 ± 8.19	7.44	112.83 ± 3.43	3.04	100.88 ± 9.39	9.30	94.98 ± 9.42	9.91
		8	105.62 ± 9.76	9.24	109.53 ± 10.14	9.25	101.00 ± 10.42	10.31	107.70 ± 11.47	10.65
		120	103.30 ± 7.75	7.50	106.00 ± 7.29	6.88	102.80 ± 6.09	5.93	101.58 ± 6.26	6.16
		1800	113.00 ± 6.13	5.43	110.83 ± 8.13	7.34	113.67 ± 8.19	7.20	113.12 ± 12.47	11.02
	Stomach	5	113.68 ± 8.42	7.41	112.13 ± 10.23	9.12	111.12 ± 10.43	9.38	110.67 ± 6.09	5.50
		8	111.67 ± 6.59	5.90	111.00 ± 5.22	4.70	109.67 ± 3.33	3.03	104.50 ± 4.71	4.51
		120	113.17 ± 3.66	3.23	111.50 ± 5.86	5.25	112.17 ± 6.15	5.48	110.92 ± 8.36	7.53
		1800	109.83 ± 7.36	6.70	111.92 ± 10.99	9.82	110.50 ± 6.28	5.69	113.83 ± 5.56	4.89
	Brain	5	109.82 ± 10.26	9.34	106.90 ± 12.09	11.31	103.05 ± 9.12	8.85	112.50 ± 5.58	4.96
		8	111.00 ± 7.42	6.52	112.50 ± 7.06	6.28	113.33 ± 3.83	3.38	111.33 ± 6.09	5.47
		120	112.50 ± 5.13	4.56	112.33 ± 6.47	5.76	113.17 ± 5.31	4.69	110.17 ± 8.28	7.52
		1800	105.82 ± 8.31	7.86	112.83 ± 7.68	6.81	110.83 ± 7.52	6.79	113.83 ± 4.49	3.95
3,4-Dimethoxyphenyl-1-O-β-apiofuroseyl(1 ⟶ 2)-β-D-glucopyranoside	Plasma	25	86.25 ± 5.40	6.26	94.95 ± 9.80	10.32	102.87 ± 10.37	10.08	105.60 ± 10.19	9.65
		50	87.20 ± 7.91	9.07	98.98 ± 11.30	11.30	94.53 ± 9.84	10.41	107.88 ± 14.96	13.87
		350	112.83 ± 4.58	4.06	112.67 ± 6.62	5.88	108.33 ± 5.13	4.73	109.07 ± 1.87	1.72
		2400	89.98 ± 4.76	5.29	89.43 ± 7.42	8.30	86.07 ± 3.39	3.93	111.74 ± 3.76	3.37
	Heart	25	98.10 ± 9.43	9.61	100.45 ± 10.89	10.85	97.55 ± 9.01	9.24	88.38 ± 5.69	6.44
		50	94.60 ± 11.14	7.80	102.70 ± 7.98	7.77	97.05 ± 8.42	8.68	99.12 ± 9.56	9.65
		350	102.57 ± 4.92	4.80	112.63 ± 98.93	7.93	110.33 ± 5.82	5.27	108.38 ± 7.34	6.77
		2400	102.23 ± 6.64	6.49	92.98 ± 8.87	9.54	101.57 ± 6.73	6.63	99.57 ± 9.46	9.50
	Liver	25	91.10 ± 10.37	11.39	102.88 ± 10.85	10.54	97.32 ± 10.44	10.73	104.70 ± 11.97	11.43
		50	105.60 ± 9.04	8.56	112.50 ± 5.65	5.02	107.48 ± 10.54	9.80	95.25 ± 11.29	11.86
		350	97.50 ± 6.63	6.80	113.00 ± 4.00	3.54	103.12 ± 5.26	5.10	108.67 ± 8.98	8.27
		2400	104.32 ± 10.18	9.76	100.42 ± 6.85	6.83	96.25 ± 5.74	5.96	107.68 ± 7.73	7.18
	Spleen	25	88.72 ± 9.93	11.19	104.47 ± 9.00	8.62	90.93 ± 9.15	10.07	112.83 ± 3.60	3.19
		50	113.17 ± 10.59	9.36	108.35 ± 10.58	9.77	95.62 ± 10.90	11.40	107.68 ± 10.39	9.65
		350	111.33 ± 7.63	6.86	111.68 ± 9.21	8.25	112.17 ± 7.99	7.12	112.17 ± 8.40	7.49
		2400	102.27 ± 11.50	11.24	112.00 ± 8.67	7.74	105.32 ± 6.59	6.26	111.00 ± 6.03	5.44
	Lung	25	90.52 ± 10.77	11.90	96.80 ± 11.29	11.67	97.07 ± 10.87	11.20	97.92 ± 11.34	11.59
		50	112.67 ± 7.84	6.96	111.02 ± 7.81	7.04	100.02 ± 12.46	12.46	109.25 ± 11.46	10.49
		350	111.33 ± 6.89	6.19	109.47 ± 11.10	10.14	103.68 ± 11.62	11.21	112.33 ± 6.92	6.16
		2400	98.85 ± 9.83	9.94	101.82 ± 8.67	8.52	100.62 ± 7.71	7.66	109.12 ± 10.69	9.79
	Kidney	25	100.05 ± 11.92	11.92	101.42 ± 8.63	8.51	96.23 ± 9.14	9.50	110.83 ± 6.24	5.63
		50	98.37 ± 7.53	7.65	102.72 ± 10.29	10.02	106.75 ± 9.15	8.57	99.88 ± 9.65	9.66
		350	90.05 ± 8.47	9.40	102.03 ± 4.61	4.52	111.83 ± 7.03	6.28	105.40 ± 10.28	9.75
		2400	104.72 ± 8.96	8.56	106.27 ± 10.19	9.59	107.85 ± 9.16	8.49	104.82 ± 8.59	8.20
	Small intestine	25	87.47 ± 7.17	8.20	96.42 ± 9.13	9.47	94.10 ± 8.05	8.55	99.25 ± 6.06	6.11
		50	109.83 ± 2.56	2.33	100.75 ± 6.11	6.07	106.83 ± 5.34	5.00	101.80 ± 8.78	8.62
		350	111.17 ± 7.73	6.95	113.67 ± 8.76	7.70	113.00 ± 3.79	3.36	106.33 ± 5.39	5.07
		2400	98.50 ± 5.49	5.57	104.65 ± 7.85	7.50	99.92 ± 5.94	5.94	94.90 ± 8.62	9.08
	Stomach	25	111.83 ± 5.78	5.17	110.50 ± 4.42	4.00	113.00 ± 6.32	5.60	106.32 ± 9.48	8.92
		50	112.75 ± 10.97	9.73	112.67 ± 3.67	3.26	112.83 ± 5.60	4.96	109.83 ± 8.66	7.88
		350	113.17 ± 3.87	3.42	108.17 ± 9.68	8.95	111.00 ± 6.20	5.58	111.83 ± 5.98	5.35
		2400	101.27 ± 7.89	7.80	94.40 ± 6.00	6.35	96.82 ± 8.08	8.34	103.08 ± 9.60	9.32
	Brain	25	99.82 ± 9.82	9.84	107.80 ± 12.79	11.86	100.68 ± 9.80	9.73	105.37 ± 7.31	6.93
		50	109.35 ± 12.15	11.11	112.83 ± 4.26	3.78	113.77 ± 10.36	9.11	113.83 ± 3.87	3.40
		350	111.50 ± 8.64	7.75	110.83 ± 7.47	6.74	111.17 ± 7.63	6.27	112.83 ± 7.78	6.90
		2400	98.95 ± 3.97	4.01	100.10 ± 9.43	9.42	98.23 ± 5.39	5.49	101.50 ± 9.94	9.80