A Simple and Sensitive HPLC-MS/MS Assay for the Quantitation of Blonanserin and N-Desethyl Blonanserin in Rat Plasma and Its Application to Pharmacokinetic Study

Table 1

Summary of intrabatch and interbatch precision and accuracy data for blonanserin and N-desethyl blonanserin in rat plasma (six replicates per level (intra) in three individual runs (inter)).

Analytes	Concentration (ng·mL^−1)	Intra (n = 6)			Inter (n = 6)
		mean ± SD (ng·mL^−1)	CV (%)	RE (%)	mean ± SD (ng·mL^−1)		CV (%)	RE (%)
Blonanserin	0.1	0.10 ± 0.01	5.28	−0.83	0.103 ± 0.02	16.11		3.17
	0.3	0.29 ± 0.01	2.44	−3.61	0.284 ± 0.02	5.57		−5.39
	30.0	29.75 ± 1.07	3.60	−0.82	29.89 ± 3.33	11.15		−0.35
	75.0	75.94 ± 2.98	3.92	1.26	77.27 ± 10.27	13.29		3.03
N-desethyl blonanserin	0.1	0.11 ± 0.017	15.84	7.33	0.103 ± 0.02	15.42		2.94
	0.3	0.28 ± 0.01	3.39	−6.61	0.283 ± 0.03	11.85		−5.52
	30.0	30.07 ± 0.29	0.96	0.25	30.78 ± 2.29	7.44		2.61
	75.0	76.03 ± 0.68	0.89	1.37	77.52 ± 6.01	6.01		3.36