A Simple and Sensitive HPLC-MS/MS Assay for the Quantitation of Blonanserin and N-Desethyl Blonanserin in Rat Plasma and Its Application to Pharmacokinetic Study

Table 3

Stability results of quality control samples in rat whole blood and plasma.

Analyte	Concentration (ng·mL^−1)	Whole blood stability (2h, n = 3)	Room temperature stability (24 h, n = 6)		Autosample stability (24 h, n = 6)		Freeze-thaw (5 cycles, n = 4)		Long term (180 d, n = 4)
		Bias (RE, %)	mean ± SD (ng·mL^−1)	Bias (RE, %)	mean ± SD (ng·mL^−1)	Bias (RE, %)	mean ± SD (ng·mL^−1)	Bias (RE, %)	mean ± SD (ng·mL^−1)	Bias (RE, %)
Blonanserin	0.3	9.28	0.30 ± 0.02	0.00	0.29 ± 0.01	−0.03	0.27 ± 0.02	−0.10	0.27 ± 0.01	−0.10
	75.0	1.66	63.86 ± 2.38	−14.85	71.58 ± 3.65	−4.56	69.61 ± 0.99	−7.19	71.63 ± 1.30	−4.49
N-desethyl blonanserin	0.3	9.08	0.28 ± 0.02	−6.67	0.27 ± 0.01	−0.10	0.29 ± 0.01	−0.03	0.28 ± 0.01	−6.67
	75.0	−6.66	67.37 ± 1.26	−10.17	74.64 ± 1.98	−0.48	75.01 ± 1.40	0.01	76.13 ± 2.10	1.51