A Sensitive Fluorescent Immunoassay for Prostate Specific Antigen Detection Based on Signal Amplify Strategy of Horseradish Peroxidase and Silicon Dioxide Nanospheres

<div>Effects of (a) pH, (b) the concentration of H2O2, (c) the ratios of HRP-Ab2-SiO2 NSs bioconjugate to L-tyrosine (v/v, L-tyrosine concentration:55 μM), and (d) the reaction time on the fluorescent intensity of the immunoassay with PSA antigen concentration of 50 ng·mL−1 (error bars represent standard deviations from three repeated measurements).</div>

Journal of Analytical Methods in Chemistry

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Figure 4

Figure 4: A Sensitive Fluorescent Immunoassay for Prostate Specific Antigen Detection Based on Signal Amplify Strategy of Horseradish Peroxidase and Silicon Dioxide Nanospheres 

Figure 4 | A Sensitive Fluorescent Immunoassay for Prostate Specific Antigen Detection Based on Signal Amplify Strategy of Horseradish Peroxidase and Silicon Dioxide Nanospheres 