UPLC-MS/MS Method for Simultaneous Determination of 14 Antimicrobials in Human Plasma and Cerebrospinal Fluid: Application to Therapeutic Drug Monitoring

Table 1

Acquisition parameters used in the UPLC-MS/MS assay.

Compounds	Parent (m/z)	Daughter (m/z)	RT (min)	Dwell (s)	Cone (V)	Collision (V)
Meropenem	384.1	141.1	0.81	0.015	27	14
Norvancomycin	718.0	144.1	0.74	0.039	30	20
Ampicillin	350.1	106.1	1.01	0.039	28	16
Linezolid	338.1	296.0	1.18	0.039	46	22
Ceftazidime	547.0	468.0	0.81	0.039	30	12
Ceftriaxone	555.0	395.9	0.93	0.039	30	12
Daptomycin	810.7	159.1	1.62	0.120	38	56
Vancomycin	725.6	144.1	0.76	0.038	35	16
Cefepime	481.0	125.0	0.73	0.038	28	52
Biapenem	351.1	110.1	0.70	0.038	34	18
Cefoperazone	647.0	530.9	1.02	0.038	35	15
Latamoxef	521.0	377.0	0.91	0.038	24	24
Etimicin	478.1	350.1	0.27	0.052	42	24
Amikacin	586.2	163.1	0.27	0.052	40	36
Linezolid-d3	341.1	296.0	1.18	0.039	46	22
Ceftriaxone-d3	558.0	395.9	0.93	0.039	30	12
Meropenem-d6	390.2	146.9	0.81	0.015	27	14

RT: retention time.