UPLC-MS/MS Method for Simultaneous Determination of 14 Antimicrobials in Human Plasma and Cerebrospinal Fluid: Application to Therapeutic Drug Monitoring

Table 2

Concentrations of calibrators (μg/mL) and regression equation.

Compounds	Calibration curve 1								Regression equation
Meropenem	0.5	1	2.5	5	10	25	50	100	Y = 3.352X − 0.0918
Norvancomycin	0.5	1	2.5	5	10	25	50	100	Y = 0.0565X − 0.00848
Ampicillin	0.5	1	2.5	5	10	25	50	100	Y = 5.671X − 2.0614
Linezolid	0.25	0.5	1.25	2.5	5	12.5	25	50	Y = 2.0248X + 0.000798
Ceftazidime	0.4	0.8	2	4	8	20	40	80	Y = 0.285X − 0.0685
Ceftriaxone	0.05	0.1	0.25	0.5	1	2.5	5	10	Y = 2.589X − 0.0144
Daptomycin	0.5	1	2.5	5	10	25	50	100	Y = 2.174X − 0.352
	Calibration curve 2
Vancomycin	0.5	0.8	2.5	5	12.5	25	50	—	Y = 0.0265X + 0.00375
Cefepime	0.5	0.8	2.5	5	12.5	25	50	—	Y = 0.0885X − 0.0232
Biapenem	0.25	0.4	1.25	2.5	6.25	12.5	25	—	Y = 0.0189X − 0.000895
Cefoperazone	0.2	0.3	1	2	5	10	20	—	Y = 0.0811X − 0.00720
Latamoxef	0.6	1	3	6	15	30	60	—	Y = 0.104X − 0.00349
Etimicin	—	0.65	2	4	10	20	40	—	Y = 0.0318X − 0.00469
Amikacin	—	1.3	4	8	20	40	80	—	Y = 0.00335X − 0.00102