UPLC-MS/MS Method for Simultaneous Determination of 14 Antimicrobials in Human Plasma and Cerebrospinal Fluid: Application to Therapeutic Drug Monitoring

Table 3

Precision and accuracy for 14 antibiotics in human plasma and cerebrospinal fluid (n = 6).

Compounds	Spiked (μg/mL)	Plasma			Cerebrospinal fluid
		Interday precision RSD (%)	Intraday accuracy (%)	Intraday precision RSD (%)	Interday precision RSD (%)	Intraday accuracy (%)	Intraday precision RSD (%)
Meropenem	1.5	2.52	109.49	2.82	2.32	99.84	3.08
	20	2.51	108.99	2.86	3.03	103.13	4.83
	80	3.34	105.49	4.59	3.91	103.37	4.15
Norvancomycin	1.5	4.72	101.77	4.14	2.88	103.00	1.67
	20	5.78	108.53	6.14	2.30	99.25	2.50
	80	4.77	105.73	3.13	1.72	108.81	2.20
Ampicillin	1.5	1.60	104.32	1.96	3.81	103.03	1.28
	20	2.36	101.77	2.36	2.39	99.69	1.50
	80	2.77	104.97	3.72	1.64	107.95	1.80
Linezolid	0.75	1.81	105.40	2.01	1.47	101.00	1.08
	10	1.63	107.17	2.35	1.41	105.77	1.05
	40	2.88	103.76	3.20	1.71	102.39	0.95
Ceftazidime	1.2	2.50	93.83	1.94	2.57	96.49	3.58
	16	2.31	98.73	2.18	3.34	101.02	4.23
	64	3.11	104.42	2.99	2.23	106.93	2.63
Ceftriaxone	0.15	2.89	108.33	3.43	5.17	99.67	5.90
	2	3.72	99.82	5.05	3.81	106.81	4.56
	8	3.84	92.15	2.98	4.79	96.58	2.44
Daptomycin	1.5	1.58	99.42	1.64	3.20	95.58	1.68
	20	1.52	110.85	2.35	1.63	107.00	1.68
	80	2.04	107.29	1.16	1.65	109.15	1.44
Vancomycin	1	8.71	101.07	10.97	6.42	103.35	7.37
	10	6.05	100.86	8.38	5.55	104.99	7.80
	40	4.97	96.11	10.53	3.69	106.54	4.46
Cefepime	1	6.14	100.57	8.22	2.87	110.85	2.64
	10	3.32	102.47	5.85	5.39	98.04	5.67
	40	3.15	103.03	1.38	2.80	110.15	3.35
Biapenem	0.5	3.17	105.30	2.56	5.07	101.57	7.07
	5	3.64	107.97	5.77	3.07	101.14	3.44
	20	3.95	101.89	5.34	2.44	96.93	2.19
Cefoperazone	0.4	3.43	97.79	2.62	2.58	107.08	2.37
	4	1.72	106.71	1.83	1.90	91.27	1.97
	16	2.83	102.19	4.26	2.63	104.84	5.26
Latamoxef	1.2	2.87	97.94	3.53	2.56	101.58	2.17
	12	1.32	109.92	1.27	2.00	104.31	2.75
	48	3.15	107.96	4.30	1.51	103.57	2.08
Etimicin	1.6	4.75	86.75	1.97	4.66	100.90	8.40
	8	2.82	87.85	3.67	3.46	104.67	2.45
	32	3.05	107.15	3.62	4.36	100.40	7.41
Amikacin	3.2	5.27	93.15	4.08	0.47	91.52	0.46
	16	6.10	103.80	7.80	2.27	89.36	1.51
	64	5.75	97.86	3.79	5.77	104.77	3.19