UPLC-MS/MS Method for Simultaneous Determination of 14 Antimicrobials in Human Plasma and Cerebrospinal Fluid: Application to Therapeutic Drug Monitoring

Table 4

Matrix effects and recoveries of 14 antibiotics in plasma and cerebrospinal fluid (n = 6).

Compounds	Spiked (μg/mL)	Plasma				Cerebrospinal fluid
		Matrix effect		Recovery		Matrix effect		Recovery
		Accuracy (%)	RSD (%)	Accuracy (%)	RSD (%)	Accuracy (%)	RSD (%)	Accuracy (%)	RSD (%)
Meropenem	1.5	81.27	11.61	64.29	7.67	64.48	11.62	64.37	12.51
	20	83.82	5.12	64.91	6.90	77.72	8.96	77.65	8.08
	80	82.03	3.39	71.97	2.51	68.24	3.58	70.21	4.65
Norvancomycin	1.5	110.29	11.66	26.40	12.19	88.92	6.56	52.07	14.20
	20	114.07	7.48	29.46	11.28	97.70	3.56	65.48	4.55
	80	114.72	1.44	31.90	6.29	89.96	5.65	67.79	5.28
Ampicillin	1.5	58.04	13.09	59.41	4.0	72.89	11.41	74.87	10.06
	20	77.29	3.17	63.72	5.11	95.01	6.30	75.11	9.65
	80	87.83	3.41	77.70	1.56	88.59	8.40	87.99	8.74
Linezolid	0.75	64.01	5.85	72.29	4.90	63.49	11.54	53.34	13.51
	10	79.68	7.23	74.53	8.43	86.76	2.04	69.91	2.32
	40	82.14	7.78	78.90	4.84	89.21	2.79	74.05	2.64
Ceftazidime	1.2	44.14	14.22	74.96	10.89	75.68	8.86	76.01	5.05
	16	45.46	2.26	69.68	6.42	93.28	14.41	84.78	13.66
	64	51.83	2.51	70.27	3.75	60.33	4.50	53.02	4.28
Ceftriaxone	0.15	53.12	11.96	49.90	12.98	62.13	14.33	66.74	14.63
	2	46.33	4.86	64.37	8.06	62.64	6.29	67.45	7.62
	8	49.55	4.11	68.12	4.56	52.86	5.38	62.25	5.94
Daptomycin	1.5	38.08	10.47	113.94	6.41	40.84	13.30	105.45	12.38
	20	43.15	4.31	95.49	2.87	65.50	7.58	106.07	8.63
	80	51.61	6.29	85.35	1.20	62.62	3.34	79.53	2.57
Vancomycin	1	81.60	10.70	70.78	6.51	51.05	10.44	39.33	12.43
	10	82.11	6.38	69.32	6.73	73.99	8.16	43.99	12.99
	40	83.84	10.38	60.81	11.87	84.62	5.44	51.32	5.96
Cefepime	1	98.78	12.58	91.97	9.23	89.68	10.32	85.12	6.75
	10	79.68	13.42	98.09	7.82	64.86	8.41	62.67	3.97
	40	90.35	4.08	86.59	6.05	73.11	2.44	113.35	3.52
Biapenem	0.5	78.30	5.53	67.67	14.46	42.65	8.01	82.90	5.96
	5	77.64	13.31	94.25	5.96	48.78	3.54	80.04	4.65
	20	67.15	4.21	76.04	9.17	60.81	1.76	89.91	2.25
Cefoperazone	0.4	69.40	6.80	77.94	12.76	83.74	6.58	107.18	4.16
	4	86.58	2.65	75.69	2.70	85.10	1.42	113.51	7.00
	16	94.99	5.31	67.85	5.05	83.88	2.33	95.48	6.39
Latamoxef	1.2	80.98	8.43	71.35	9.40	87.96	13.65	51.56	11.00
	12	97.79	3.14	64.59	4.28	108.96	13.99	73.15	14.59
	48	99.55	1.58	66.27	3.45	103.79	10.47	62.15	8.75
Etimicin	1.6	98.00	8.78	75.25	6.85	98.89	6.01	100.38	9.18
	8	94.96	3.52	94.80	8.51	113.02	3.10	97.25	3.16
	32	94.32	4.44	88.27	5.37	83.48	2.65	114.49	2.76
Amikacin	3.2	99.91	3.68	107.03	13.64	90.74	10.96	98.87	2.53
	16	87.05	7.32	114.77	6.48	70.94	8.04	100.45	3.99
	64	99.56	7.76	110.16	10.32	99.41	2.29	99.31	2.37