Qualitative and Quantitative Evaluation of Chemical Constituents from Shuanghuanglian Injection Using Nuclear Magnetic Resonance Spectroscopy

Table 3

Summary results of linear regression, LODs, LOQs, precision, repeatability, stability, and recovery for the primary metabolites in SHLI.

Primary metabolites	Linear regression			Precision RSD (%)		Repeatability (n = 6, RSD, %)	Stability (n = 6, RSD, %)	Recovery (n = 6, mean ± SD, %)
	Regression equation	Linear range (mg/mL)	Correlation coefficients (r)	Intraday	Interday
Valine	y = 274.19x − 0.0613	0.0010–0.1320	0.9998	1.3	1.0	0.8	1.2	98.43 ± 1.55
Glucose	y = 60.268x − 2.3468	0.0540–6.9180	0.9998	0.6	0.5	1.9	0.3	100.94 ± 1.74
Fructose	y = 123.09x − 6.5731	0.0696–8.9108	0.9997	0.4	0.6	0.3	0.6	102.30 ± 1.52
Mannose	y = 53.151x + 0.3726	0.0053–0.6840	0.9999	1.1	1.3	2.7	0.7	105.93 ± 0.52
Sucrose	y = 31.521x − 0.1564	0.0167–2.1320	0.9998	0.4	0.5	1.1	0.5	93.96 ± 1.14
Succinic acid	y = 364.01x − 0.1605	0.0008–0.0961	0.9997	0.4	0.7	0.6	0.4	102.66 ± 1.69
myo-Inositol	y = 85.074x − 1.9043	0.0288–3.6924	0.9997	0.7	0.7	0.4	0.4	135.78 ± 1.21