Semiconducting Carbon Nanotube-Based Nanodevices for Monitoring the Effects of Chlorphenamine on the Activities of Intracellular Ca²⁺ Stores

Figure 3

Real-time monitoring of the electrophysiological activities of a HeLa cell under histamine stimulation. (a) Schematic drawing depicting the mechanism of Ca²⁺ release from intracellular stores of HeLa cells stimulated by histamine: (i) resting state of a HeLa cell; (ii) stimulation of H1Rs by histamine resulting in the Ca²⁺ release from intracellular stores. (b) Schematic drawing depicting the effects of chlorphenamine on histamine-induced Ca²⁺ release from intracellular stores: (i) inactivation of H1Rs by chlorphenamine; (ii) reduction of histamine-induced Ca²⁺ release caused by a cell pretreatment with chlorphenamine. (c) Real-time monitoring of the conductance changes of a SCN device (i) without a HeLa cell, (ii) with a chlorphenamine nontreated HeLa cell, and (iii) with a chlorphenamine pretreated HeLa cell during the addition of 100 μM histamine.