Self-Renewal Signalling in Presenescent Tetraploid IMR90 Cells
Figure 7
Examples of DNA content measurements by integral fluorescence in the DAPI channel using Image Pro Plus software in microscopic fields including cells with enlarged nuclei and stained for: ((a), (b)) NANOG, p16, and DNA by DAPI, ((c), (d)) Aurora B-kinase and DNA by DAPI. The selected hyperoctoploid cell (*) shown in (a) has an enlarged p16-positive cytoplasm and some amount of both NANOG and p16 in the nuclear region, and this region is magnified in the insertion; the paraoctoploid cell in (c) is positive for Aurora B-kinase.
