Protein deacetylation by Sirtuins. Sirtuins deacetylate lysine (K) residues of target proteins using cofactor—Nicotinamide-adenine-dinucleotide (NAD+) and releasing Nicotinamide (NAM). 2′-O-acetyl-ADP ribose is generated as a result of transfer of the acetyl group of K onto ADP-ribose residue. Deacetylation is inhibited by NAM, which can also reverse the reaction to reproduce NAD+.
