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Journal of Aging Research
Volume 2011, Article ID 908035, 9 pages
Review Article

Proteomic Profiling of Mitochondrial Enzymes during Skeletal Muscle Aging

1Department of Biology, National University of Ireland, Maynooth, County Kildare, Ireland
2National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Ireland

Received 21 September 2010; Revised 17 November 2010; Accepted 3 January 2011

Academic Editor: Alberto Sanz

Copyright © 2011 Lisa Staunton et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Mitochondria are of central importance for energy generation in skeletal muscles. Expression changes or functional alterations in mitochondrial enzymes play a key role during myogenesis, fibre maturation, and various neuromuscular pathologies, as well as natural fibre aging. Mass spectrometry-based proteomics suggests itself as a convenient large-scale and high-throughput approach to catalogue the mitochondrial protein complement and determine global changes during health and disease. This paper gives a brief overview of the relatively new field of mitochondrial proteomics and discusses the findings from recent proteomic surveys of mitochondrial elements in aged skeletal muscles. Changes in the abundance, biochemical activity, subcellular localization, and/or posttranslational modifications in key mitochondrial enzymes might be useful as novel biomarkers of aging. In the long term, this may advance diagnostic procedures, improve the monitoring of disease progression, help in the testing of side effects due to new drug regimes, and enhance our molecular understanding of age-related muscle degeneration.