Blockade of Electron Transport at the Onset of Reperfusion Decreases Cardiac Injury in Aged Hearts by Protecting the Inner Mitochondrial Membrane
Figure 4
Measurement of mitochondrial inner membrane potential. The upper panel is an original tracing of an inner membrane potential measurement. Glutamate (10 mM) was used as a complex I substrate to polarize the inner membrane potential. ADP stimulated the depolarization of inner membrane potential. Inner membrane potential was restored when oligomycin was used to inhibit complex V. Dinitrophenol (DNP) was titrated to completely collapse inner membrane potential. The Δ change of fluorescence intensity was used to assess inner mitochondrial membrane potential in the presence of ADP, oligomycin, and DNP. The lower panel provides representative tracings of inner membrane potential from mitochondria from untreated (blue line) and amobarbital-treated (red line) hearts.
