Research Article

Extension of Chronological Lifespan by Hexokinase Mutation in Kluyveromyces lactis Involves Increased Level of the Mitochondrial Chaperonin Hsp60

Figure 2

Evaluation of gene dosage of RAG5 in determining the lifespan. Comparison of chronological survival in YNB medium of wt and mutant lacking RAG5 and the same strain overexpressing RAG5. Survival was assessed in minimal medium for plasmid maintenance. Over time, aliquots of cultures were counted and serial dilutions were plated on solid medium. Colony forming units were counted after 3 days. Data are representative of five independent experiments, mean ± s.d.
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