The Cooccurrence of Obesity, Osteoporosis, and Sarcopenia in the Ovariectomized Rat: A Study for Modeling Osteosarcopenic Obesity in Rodents

Table 4

The effects of ovariectomy (ovx) and 17β-estradiol (ovx + E₂) on muscle mass and normalized muscle mass in rats with initial ages of five and ten months. Ovariectomy did not affect the muscle mass but decreased ratio of tibial bone mineral content to combined muscle mass in both age groups and lowered normalized values of gastrocnemius muscle to body weight in older rats that were prevented by E₂ administration.

Parameters	5-month-old			10-month-old
	Sham	Ovx	Ovx + E2	Sham	Ovx	Ovx + E2
Muscle mass
Gastrocnemius (g)	1.7814 ± 5.886	2.0763 ± 4.442	1.8828 ± 5.747	2.228 ± 1.09	2.242 ± 0.004	2.129 ± 0.054
Soleus (mg)	135 ± 9.2	163 ± 9.1	139 ± 12	150 ± 7.0	151 ± 9.1	140 ± 5.1
MM/BW (mg/g)
Gastrocnemius	4.881 ± 0.0231	5.191 ± 0.0118	5.458 ± 0.0197	5.28 ± 0.005	4.73 ± 0.011	5.28 ± 0.015
Soleus	0.401 ± 0.029	0.408 ± 0.024	0.402 ± 0.034	0.37 ± 0.001	0.31 ± 0.02	0.34 ± 0.003
BMC/CMM (μg/mg)	16.483 ± 0.596	13.042 ± 0.125	14.872 ± 0.400	15.728 ± 0.795	13.879 ± 0.304	14.541 ± 0.439

Values are means ± SEM. Values that do not share the same superscript letters in each age category are significantly () different from each other. MM, muscle mass; BW, body weight, BMC, right tibia bone mineral content; CMM, combined muscle mass ( per treatment group).