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Journal of Aging Research
Volume 2017, Article ID 9851380, 11 pages
https://doi.org/10.1155/2017/9851380
Research Article

Knockout of Vasohibin-1 Gene in Mice Results in Healthy Longevity with Reduced Expression of Insulin Receptor, Insulin Receptor Substrate 1, and Insulin Receptor Substrate 2 in Their White Adipose Tissue

1Department of Vascular Biology, Institute of Development, Aging, and Cancer, Tohoku University, Sendai, Japan
2Department of Diabetes and Metabolism, Graduate School of Medicine, Tokyo University, Tokyo, Japan

Correspondence should be addressed to Yasufumi Sato; pj.ca.ukohot@3b.otas.imufusay

Received 13 December 2016; Revised 30 January 2017; Accepted 16 February 2017; Published 6 March 2017

Academic Editor: Barbara Shukitt-Hale

Copyright © 2017 Eichi Takeda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Vasohibin-1 (Vash1), originally isolated as an endothelium-derived angiogenesis inhibitor, has a characteristic of promoting stress tolerance in endothelial cells (ECs). We therefore speculated that the lack of the vash1 gene would result in a short lifespan. However, to our surprise, vash1−/− mice lived significantly longer with a milder senescence phenotype than wild-type (WT) mice. We sought the cause of this healthy longevity and found that vash1−/− mice exhibited mild insulin resistance along with reduced expression of the insulin receptor (insr), insulin receptor substrate 1 (irs-1), and insulin receptor substrate 2 (irs-2) in their white adipose tissue (WAT) but not in their liver or skeletal muscle. The expression of vash1 dominated in the WAT among those 3 organs. Importantly, vash1−/− mice did not develop diabetes even when fed a high-fat diet. These results indicate that the expression of vash1 was required for the normal insulin sensitivity of the WAT and that the target molecules for this activity were insr, irs1, and irs2. The lack of vash1 caused mild insulin resistance without the outbreak of overt diabetes and might contribute to healthy longevity.