Knockout of Vasohibin-1 Gene in Mice Results in Healthy Longevity with Reduced Expression of Insulin Receptor, Insulin Receptor Substrate 1, and Insulin Receptor Substrate 2 in Their White Adipose Tissue

Figure 4

No alteration in the expression of tnfα, sod2, and sirt1 in the WAT of young vash1^−/− mice. (a) Total RNA was isolated from the WT and vash1^−/− WAT of young male mice, and the expression of tnfα was compared between WT and vash1^−/− mice. (b) Total RNA was isolated from WAT of WT and vash1^−/− of young male mice, and the expression of sod2 and sirt1 was compared between WT and vash1^−/− mice. In (a) and (b), the means ± SDs are shown, the statistical significance of differences was calculated by use of the unpaired Student’s -test, and a value of was taken as the criterion for significance.

(a)

(b)