Knockout of Vasohibin-1 Gene in Mice Results in Healthy Longevity with Reduced Expression of Insulin Receptor, Insulin Receptor Substrate 1, and Insulin Receptor Substrate 2 in Their White Adipose Tissue

Figure 5

Vash1 is expressed in ECs but not in adipocytes. (a) CD31-positive and -negative cells from the WAT of young WT male mice were separated as described in Materials and Methods, and the expression of vash1 was compared. Means ± SDs are shown. The statistical significance of differences was calculated by use of the unpaired Student’s -test, and a value of was the criterion for significance. (b) 3T3L1 preadipocytes were caused to differentiate into adipocyte in vitro, and the expression of vash1 in those cells was quantified as described in Materials and Methods. .

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